ISOTOPIC EXCHANGE PLUS SUBSTRATE AND INHIBITION-KINETICS OF D-XYLOSE ISOMERASE DO NOT SUPPORT A PROTON-TRANSFER MECHANISM
ISOTOPIC EXCHANGE PLUS SUBSTRATE AND INHIBITION-KINETICS OF D-XYLOSE ISOMERASE DO NOT SUPPORT A PROTON-TRANSFER MECHANISM
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DOI：
10.1021/bi00172a026
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发表时间：
1994-02-15
期刊：
影响因子：
2.9
通讯作者：
RINGE, D
中科院分区：
文献类型：
--
作者：
ALLEN, KN;LAVIE, A;RINGE, D
橄榄产色链霉菌的D-木糖异构酶是一种Mg 2+或Mn 2+依赖性酶，其催化木糖至木酮糖或葡萄糖至果糖的醛糖-酮糖异构化。在15、25和55 ℃下，在C-2氚化葡萄糖的酶催化异构化过程中，质子交换到水中，
The D-xylose isomerase of Streptomyces olivochromogenes is a Mg2+- or Mn2+-dependent enzyme that catalyzes the aldose-ketose isomerization of xylose to xylulose or of glucose to fructose. Proton exchange into water during enzyme-catalyzed isomerization of C-2 tritiated glucose at 15, 25 and 55 degrees C shows