Genetic and microbial diversity of the invasive mosquito vector species Culex tritaeniorhynchus across its extensive inter-continental geographic range-猫眼课题宝

DOI：

10.1101/2022.02.10.479990

发表时间：

2022-02

期刊：

bioRxiv

影响因子：

通讯作者：

中科院分区：

其他

文献类型：

作者：

C. Jeffries;L. M. Tantely;P. Kadriaj;Marcus S. C. Blagrove;I. Lytra;J. Orsborne;H. M. Al-Amin;A. Mohammed;M. Alam;R. Girod;Y. Afrane;S. Bino;V. Robert;S. Boyer;M. Baylis;E. Velo;G. Hughes;Thomas Walker

库蚊（Cx.）三带喙库蚊是一种具有广泛和不断扩大的洲际地理分布的蚊子，目前在亚洲、非洲、中东、欧洲和现在的澳大利亚的50多个国家都有报道。它是引起医学和兽医关注的重要病媒，能够传播多种虫媒病毒，在人类和动物群体中造成重大发病率和死亡率。在亚洲流行性乙型脑炎病毒（JEV）流行的区域，Cx。三带喙蚊被认为是主要的病媒，该物种也被证明有助于传播其他几种重要的人畜共患虫媒病毒，包括裂谷热病毒和西尼罗河病毒。在不同的病媒种群之间，病媒能力可能存在显著差异。了解一个物种在其地理范围内的情况对于了解其在不同环境和地区传播病原体的重要性至关重要。媒介能力可受到多种因素的影响，包括蚊子的遗传背景、与蚊子有关的微生物群组成以及人类或动物病原体的共同感染。除了加强关于病媒监测和病原体传播潜在风险的信息外，确定病媒物种不同种群的遗传和微生物多样性对于制定和应用有效的控制战略也至关重要。在本研究中，多个地理上分散的Cx种群。采集了来自欧洲、非洲、欧亚和亚洲各国的三带喙蚊样本。分子分析表明，蚊子种群内部和种群之间存在高度的遗传和微生物多样性，包括蚊子CO1基因的遗传差异，以及16S rRNA基因扩增子测序鉴定出的多种微生物组。利用沃尔巴克氏体特异性PCR检测和沃尔巴克氏体MLST基因测序，证实了在一些种群中检测到内共生细菌沃尔巴克氏体的证据；除了基于聚合酶链反应的昆虫特异性病毒检测。实验室媒介能力显示Cx。来自希腊种群的三带喙蚊可能是乙脑病毒的有效媒介。本研究扩大了对Cx多样性的认识。三带喙蚊在其洲际范围内的分布，突出了对这种入侵病媒物种的更多关注的必要性，并有助于为病媒控制策略的发展提供潜在的未来方向。

Culex (Cx.) tritaeniorhynchus is a mosquito species with an extensive and expanding inter-continental geographic distribution, currently reported in over 50 countries, across Asia, Africa, the Middle East, Europe and now Australia. It is an important vector of medical and veterinary concern, capable of transmitting multiple arboviruses which cause significant morbidity and mortality in human and animal populations. In regions endemic for Japanese encephalitis virus (JEV) in Asia, Cx. tritaeniorhynchus is considered the major vector and this species has also been shown to contribute to the transmission of several other significant zoonotic arboviruses, including Rift Valley fever virus and West Nile virus. Significant variation in vectorial capacity can occur between different vector populations. Obtaining knowledge of a species from across its geographic range is crucial to understanding its significance for pathogen transmission across diverse environments and localities. Vectorial capacity can be influenced by factors including the mosquito genetic background, composition of the microbiota associated with the mosquito and the co-infection of human or animal pathogens. In addition to enhancing information on vector surveillance and potential risks for pathogen transmission, determining the genetic and microbial diversity of distinct populations of a vector species is also critical for the development and application of effective control strategies. In this study, multiple geographically dispersed populations of Cx. tritaeniorhynchus from countries within Europe, Africa, Eurasia and Asia were sampled. Molecular analysis demonstrated a high level of genetic and microbial diversity within and between populations, including genetic divergence in the mosquito CO1 gene, as well as diverse microbiomes identified by 16S rRNA gene amplicon sequencing. Evidence for the detection of the endosymbiotic bacteria Wolbachia in some populations was confirmed using Wolbachia-specific PCR detection and sequencing of Wolbachia MLST genes; in addition to PCR-based detection of insect-specific viruses. Laboratory vector competence showed Cx. tritaeniorhynchus from a Greek population are likely to be competent vectors of JEV. This study expands understanding of the diversity of Cx. tritaeniorhynchus across its inter-continental range, highlights the need for a greater focus on this invasive vector species and helps to inform potential future directions for development of vector control strategies.

Genetic and microbial diversity of the invasive mosquito vector species Culex tritaeniorhynchus across its extensive inter-continental geographic range

文献摘要

相似文献