QUANTITATIVE-ANALYSIS OF THE HYDROGEN-PEROXIDE FORMED IN AQUEOUS CIGARETTE TAR EXTRACTS
QUANTITATIVE-ANALYSIS OF THE HYDROGEN-PEROXIDE FORMED IN AQUEOUS CIGARETTE TAR EXTRACTS
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DOI：
10.1016/0891-5849(89)90094-4
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发表时间：
1989-01-01
影响因子：
7.4
通讯作者：
PRYOR, WA
中科院分区：
文献类型：
--
作者：
NAKAYAMA, T;CHURCH, DF;PRYOR, WA
我们首次建立了一种可靠的方法来定量测定香烟焦油水提取物中产生的过氧化氢（H2O2）。通过短反相柱，用自动参比减法系统差脉冲极谱法测定沥青水提物的H2O2浓度。H2O2浓度随老化、pH和温度的升高而升高，超氧化物歧化酶的存在导致H2O2浓度降低。该方法已应用于多种研究和商业卷烟。除了少数例外，每根香烟烟雾混合一段时间后形成的H2O2量与其焦油产量成正比。
We have established, for the first time, a reliable method to quantitate hydrogen peroxide (H2O2) generated in aqueous extracts of cigarette smoker tar. The aqueous tar extract was passed through a short reverse-phase column and its H2O2 concentration determined by differential pulse polarography using an automatic reference substraction system. The H2O2 concentration increased with aging, pH and temperature: the presence of superoxide dismutase lead to lower H2O2 concentations. This method was applied to many kinds of research and commercial cigarettes. With a few exceptions, the amount of H2O2 formed after a mixed time from each cigarette smoke was proportional to its tar yield.