X-irradiation effects on growth and metamorphosis of gastropod larvae (Crepidula fornicata): a model for environmental radiation teratogenesis.
X-irradiation effects on growth and metamorphosis of gastropod larvae (Crepidula fornicata): a model for environmental radiation teratogenesis.
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X 射线对腹足动物幼虫（Crepidula fornicata）生长和变态的影响：环境辐射致畸模型。
DOI：
10.1007/bf01059971
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发表时间：
1986
影响因子：
4
通讯作者：
FitzGerald,TJ
中科院分区：
文献类型：
--
作者：
Greenberger,JS;Pechenik,J;Lord,A;Gould,L;Naparstek,E;Kase,K;FitzGerald,TJ
关于x射线照射对多细胞海洋生物发育的影响的资料很少。以200 rad/min， 250 kVp的x射线照射海洋腹足crepidula fornicataa的幼虫，每次照射剂量在500至20,000 rad之间。在暴露后的几周内，测量了壳长和生物量的变化、幼年期变态发生率和死亡率。20天的研究结果表明，剂量超过2000 rad后，幼虫壳的生长速度呈剂量依赖性下降（第20天对照=850±110 μm长度，500 rad 820±11 μm, 2000 rad 750±30 μm, 5000 rad 710±30 μm, 10,000 rad 620±30 μm, 20,000 rad 580±15 μm）。当剂量超过10,000 rad时，壳长特异性生物量显著减少。当剂量超过2,000 rad时，发现幼虫死亡率显著增加。暴露于5,000 rad时，幼虫变态的累积百分比下降，早在照射后18天就可检测到；然而，5000 rad后的幼虫在第21天就发生了更快的变态，而其他组的变态需要34-35天才能完成。为研究x射线对多细胞生物的致畸作用提供了一个非常灵敏、方便的系统。
Little information is available on the effects of x-irradiation on the development of multicellular marine organisms. Larvae of the marine gastropodCrepidula fornicatawere irradiated at 200 rad/min, 250 kVp x-rays, to doses between 500 and 20,000 rad in a single fraction. During the weeks following exposure, changes in shell length and biomass, incidence of metamorphosis to the juvenile stage of development, and mortality were measured. The results over a 20-day period demonstrated a dose-dependent decrease in growth rate of larval shells following doses above 2,000 rad (control at day 20=850±110 μm length, 820±11 μm for 500 rad, 750±30 μm for 2,000 rad, 710±30 μm for 5,000 rad, 620±30 μm for 10,000 rad, and 580±15 μm for 20,000 rad). Shell length-specific biomass was significantly decreased for doses above 10,000 rad. A significant increase in larval mortality was detected with doses above 2,000 rad. The cumulative percent of larval metamorphosis was decreased by exposures to 5,000 rad and was detectable as early as 18 days after irradiation; however, metamorphosis of larvae after 5,000 rad occurred faster by day 21 while other groups metamorphosis required 34–35 days for completion.Crepidula fornicatamay provide a very sensitive and convenient system in which to study teratogenic effects of x-irradiation on multicellular organisms.