Preparation, mechanical property and cytocompatibility of poly(L-lactic acid)/calcium silicate nanocomposites with controllable distribution of calcium silicate nanowires
Preparation, mechanical property and cytocompatibility of poly(L-lactic acid)/calcium silicate nanocomposites with controllable distribution of calcium silicate nanowires
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DOI：
10.1016/j.actbio.2012.07.009
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发表时间：
2012-11-01
影响因子：
9.7
通讯作者：
Chang, Jiang
中科院分区：
文献类型：
--
作者：
Dou, Yuandong;Wu, Chengtie;Chang, Jiang
如何精确控制生物活性无机/有机纳米复合材料的微观结构仍然是一个重大的挑战，这对影响其机械强度和生物性能具有重要意义。在这项研究中，使用静电纺丝和热压处理相结合的方法，硅酸钙水合物（CSH）纳米线/聚（L-乳酸）（PLLA）纳米复合材料的可控的微观结构和定制的机械性能，成功地制备作为潜在的骨移植替代品。将具有不同取向度的电纺混合纳米纤维以预定的方式堆叠在一起，并热压成分级结构的纳米复合材料。系统地评价了所制备的纳米复合材料的微观结构与力学性能之间的关系。结果表明，在PLEA基体中，CSH纳米线能够从完全随机取向到单轴取向，然后以不同的层间角度分级组织，从而获得相应的纳米复合材料，具有改善的力学性能和不同的各向异性。另外还发现，添加5 wt.%的纳米复合材料的弯曲强度比未添加5 wt.%的纳米复合材料的弯曲强度高。CSH纳米线（130 MPa）的拉伸强度显著高于纯PLLA（86 MPa）和其它复合材料。CSH纳米线的加入大大增强了PLLA膜的亲水性和磷灰石形成能力，以及骨髓基质细胞的附着和增殖。该研究表明，静电纺丝和热压的组合是一种可行的手段，以控制微观结构和力学性能，并提高矿化能力和细胞反应，CSH/PLLA纳米复合材料的潜在骨修复应用。皇冠版权所有（C）2012由爱思唯尔有限公司代表Acta Materialia Inc.出版。All rights reserved.
How to accurately control the microstructure of bioactive inorganic/organic nanocomposites still remains a significant challenge, which is of great importance in influencing their mechanical strength and biological properties. In this study, using a combined method of electrospinning and hot press processing, calcium silicate hydrate (CSH) nanowire/poly(L-lactide) (PLLA) nanocomposites with controllable microstructures and tailored mechanical properties were successfully prepared as potential bone graft substitutes. The electrospun hybrid nanofibers with various degrees of alignment were stacked together in a predetermined manner and hot pressed into hierarchically structured nanocomposites. The relationship between the microstructure and mechanical properties of the as-prepared nanocomposites were systematically evaluated. The results showed that CSH nanowires in a PLEA matrix were able to be controlled from completely randomly oriented to uniaxially aligned, and then hierarchically organized with different interlayer angles, leading to corresponding nanocomposites with improved mechanical properties and varied anisotropies. It was also found that the bending strength of nanocomposites with 5 wt.% CSH nanowires (130 MPa) was significantly higher than that of pure PLLA (86 MPa) and other composites. The addition of CSH nanowires greatly enhanced the hydrophilicity and apatite-forming ability of PLLA films, as well as the attachment and proliferation of bone marrow stromal cells. The study suggested that a combination of electrospinning and hot pressing is a viable means to control the microstructure and mechanical properties, and improve the mineralization ability and cellular responses, of CSH/PLLA nanocomposites for potential bone repair applications. Crown Copyright (C) 2012 Published by Elsevier Ltd. on behalf of Acta Materialia Inc. All rights reserved.