Robust, microfabricated culture devices with improved control over the soluble microenvironment for the culture of embryonic stem cells.
Robust, microfabricated culture devices with improved control over the soluble microenvironment for the culture of embryonic stem cells.
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DOI：
10.1002/biot.201300245
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发表时间：
2014-06
影响因子：
4.7
通讯作者：
Szita N
中科院分区：
文献类型：
--
作者：
Macown RJ;Veraitch FS;Szita N
干细胞的商业用途继续受到开发有效和可靠的生产方案的困难和高成本的限制。微加工系统的使用将对细胞微环境的良好控制与过程优化中资源的减少使用相结合。我们先前报道的微制造培养装置被证明适用于胚胎干细胞的培养，但需要改进鲁棒性、易用性和溶解气体控制。在本报告中，我们描述了对微加工系统设计的许多改进，以显着改善对剪切应力和可溶性因素（特别是溶解氧）的控制。这些控制改进的有限元建模进行了研究。设计上的改进也使系统更易于使用，并提高了鲁棒性。该培养装置可应用于多能干细胞生长和分化的优化，以及各种规模的监测和控制策略和改进的培养系统的开发。
The commercial use of stem cells continues to be constrained by the difficulty and high cost of developing efficient and reliable production protocols. The use of microfabricated systems combines good control over the cellular microenvironment with reduced use of resources in process optimization. Our previously reported microfabricated culture device was shown to be suitable for the culture of embryonic stem cells but required improvements to robustness, ease of use, and dissolved gas control. In this report, we describe a number of improvements to the design of the microfabricated system to significantly improve the control over shear stress and soluble factors, particularly dissolved oxygen. These control improvements are investigated by finite element modeling. Design improvements also make the system easier to use and improve the robustness. The culture device could be applied to the optimization of pluripotent stem cell growth and differentiation, as well as the development of monitoring and control strategies and improved culture systems at various scales.