Composite organic-inorganic nanoparticles as Raman labels for tissue analysis
Composite organic-inorganic nanoparticles as Raman labels for tissue analysis
复制标题
DOI：
10.1021/nl062453t
复制
发表时间：
2007-02-01
期刊：
影响因子：
10.8
通讯作者：
Knudsen, Beatrice
中科院分区：
文献类型：
--
作者：
Sun, Lei;Sung, Kung-Bin;Knudsen, Beatrice
复合有机 - 无机纳米粒子（COINs）是用于检测生物分子的新型光学标记物。我们之前已经开发了封装COINs以及用抗体对其进行功能化的方法。在此，我们报告了将COINs应用于检测人体组织中蛋白质的初步步骤。在直接结合测定中，使用两种不同的COINs同时检测两种分析物，即前列腺特异性抗原（PSA）和细胞角蛋白18（CK18），并且表明两种不同的COINs可同时标记组织样本中的PSA。
Composite organic-inorganic nanoparticles (COINs) are novel optical labels for detection of biomolecules. We have previously developed methods to encapsulate COINs and to functionalize them with antibodies. Here we report the first steps toward application of COINs to the detection of proteins in human tissues. Two analytes, PSA and CK18, are detected simultaneously using two different COINs in a direct binding assay, and two different COINs are shown to simultaneously label PSA in tissue samples.