In vivo effects on intron retention and exon skipping by the U2AF large subunit and SF1/BBP in the nematode Caenorhabditis elegans.
In vivo effects on intron retention and exon skipping by the U2AF large subunit and SF1/BBP in the nematode Caenorhabditis elegans.
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DOI：
10.1261/rna.027458.111
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发表时间：
2011-12
期刊：
影响因子：
4.5
通讯作者：
Long Ma;Zhiping Tan;Y. Teng;S. Hoersch;H. Horvitz
中科院分区：
文献类型：
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作者：
Long Ma;Zhiping Tan;Y. Teng;S. Hoersch;H. Horvitz
在动物体内分析剪接因子在调节可变剪接中的作用仍然是一个挑战。使用基于微阵列的屏幕，我们确定了秀丽隐杆线虫基因，tos-1，表现出三个四种主要类型的选择性剪接：内含子保留，外显子跳跃，并在U2 AF大亚基突变的存在下，使用选择性3'剪接位点。剪接因子U2 AF大亚基和SF 1/BBP的突变改变了tos-1的剪接。保留内含子的3'剪接位点或跳跃外显子之前的剪接位点调节TOS-1的剪接模式。我们的研究提供了在体内的证据，内含子保留和外显子跳跃可以调节很大程度上由3'剪接位点的身份。
The in vivo analysis of the roles of splicing factors in regulating alternative splicing in animals remains a challenge. Using a microarray-based screen, we identified a Caenorhabditis elegans gene, tos-1, that exhibited three of the four major types of alternative splicing: intron retention, exon skipping, and, in the presence of U2AF large subunit mutations, the use of alternative 3' splice sites. Mutations in the splicing factors U2AF large subunit and SF1/BBP altered the splicing of tos-1. 3' splice sites of the retained intron or before the skipped exon regulate the splicing pattern of tos-1. Our study provides in vivo evidence that intron retention and exon skipping can be regulated largely by the identities of 3' splice sites.