Preventive effect of natural and synthetic antioxidants on lipid peroxidation in the mammalian eye.
Preventive effect of natural and synthetic antioxidants on lipid peroxidation in the mammalian eye.
复制标题
DOI：
10.1159/000267901
复制
发表时间：
1996
影响因子：
2.1
通讯作者：
Takako Ueda;T. Ueda;Donald Armstrong
中科院分区：
文献类型：
--
作者：
Takako Ueda;T. Ueda;Donald Armstrong
用5 mM的铁和等分的牛或猪视网膜、纯化的杆状外段（ROS）和视网膜色素上皮（RPE）进行脂质过氧化。氧化30分钟后，组织中脂质过氧化氢含量比对照水平增加了约4- 8倍，其中ROS变化最大，RPE最低。研究了8种天然或合成的脂质和水性抗氧化剂以及5种黄酮类化合物对该反应的抑制能力。在所研究的部分中观察到物种差异。棕榈油来源的维生素E对牛视网膜、ROS和RPE具有显著的保护作用。在猪眼中，维生素E也提供了主要的防御，但MCLA和其他化合物如EPC-K1和U74500A在10(-5)M浓度下都很活跃。在测试的黄酮类化合物中，发现在10(-5)M水平时，牛视网膜对表没食子儿茶素没食子酸酯（EGCg）、槲皮素、薯蓣皂苷和碧萝酚有部分保护作用。当维生素E与EGCg或槲皮素联合使用时，个体效应增强。这些结果证明了体外模型系统的有效性，该系统可以快速准确地确定抗氧化剂抗脂质过氧化的能力。
Lipid peroxidation was initiated with 5 mM ferric iron and aliquots of bovine or porcine retina, purified rod outer segments (ROS) and retinal pigment epithelium (RPE). After 30 min of oxidation, the lipid hydroperoxide content of the tissues increased approximately 4- to 8-fold over control levels with ROS showing the greatest change and RPE the lowest. Eight lipid and aqueous antioxidants from natural or synthetic sources and five flavonoids were evaluated for their ability to inhibit the reaction. Species differences were observed with regard to the fraction under study. Palm oil-derived vitamin E showed significant protection in bovine retina, ROS and RPE. In the porcine eye, the major defense was also afforded by vitamin E, but MCLA and other compounds such as EPC-K1 and U74500A were all quite active at 10(-5) M concentration. Of the flavonoids tested, partial protection in the bovine retina was found at 10(-5) M levels for epigallocatechin gallate (EGCg), quercetin, diosmetin and pycnogenol. When vitamin E and EGCg or quercetin were combined, the individual effect was enhanced. These results demonstrate the usefulness of an in vitro model system that can rapidly and accurately determine the capacity of antioxidants against lipid peroxidation.