Multi-wavelength immunoassays using surface plasmon-coupled emission
Multi-wavelength immunoassays using surface plasmon-coupled emission
复制标题
DOI：
10.1016/j.bbrc.2003.12.010
复制
发表时间：
2004-01-16
影响因子：
3.1
通讯作者：
Lakowicz, JR
中科院分区：
文献类型：
--
作者：
Matveeva, E;Malicka, J;Lakowicz, JR
我们描述了一种利用表面等离子体耦合发射（SPCE）进行多波长免疫分析的新方法。这种现象是受激发的荧光团与附近的金属薄膜耦合，在我们的例子中是银，导致强烈的定向发射到底层的玻璃衬底。辐射通过棱镜传播的角度取决于相关波长的表面等离子体角度。这些角度取决于发射波长，允许使用多个发射波长测量多个分析物。我们用罗丹明Red-X或AlexaFluor 647标记的抗体证明了这种可能性。这些抗体直接针对结合在银表面的抗原蛋白。每个标记抗体的发射在玻璃棱镜上以不同的角度发生，允许独立测量每个抗体的表面结合。这种方法的SPCE免疫分析可以很容易地扩展到4个或更多的波长。(C) 2003 Elsevier Inc.版权所有。
We describe a new method for multi-wavelength immunoassays using surface plasmon-coupled emission (SPCE). This phenomenon is coupling of excited fluorophores with a nearby thin metal film, in our case silver, resulting in strongly directional emission into the underlying glass substrate. The angle at which the radiation propagate through the prism depends on the surface plasmon angle for the relevant wavelength. These angles depend on emission wavelength, allowing measurement of multiple analytes using multiple emission wavelengths. We demonstrated this possibility using antibodies labeled with either Rhodamine Red-X or AlexaFluor 647. These antibodies were directed against an antigen protein bound to the silver surface. The emission from each labeled antibody occurred at a different angle on the glass prism, allowing independent measurement of surface binding of each antibody. This method of SPCE immunoassays can be readily extended to 4 or more wavelengths. (C) 2003 Elsevier Inc. All rights reserved.