[Experimental studies on the penetration of dermatophytes into the human stratum corneum and the effects of washing].
[Experimental studies on the penetration of dermatophytes into the human stratum corneum and the effects of washing].
复制标题
皮肤癣菌侵入人体角质层及洗涤效果的实验研究
DOI：
10.3314/jjmm.45.247
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
I. Takiuchi
中科院分区：
文献类型：
--
作者：
N. Morishita;J. Ninomiya;Y. Sei;I. Takiuchi
在将几种类型的皮肤真菌应用于从健康人脚跟获得的角质层后，我们评估了真菌成分进入角质层的渗透速度和洗涤其表面的效果。我们设计了以下两种环境，假设在将真菌元素应用于角质层表面后，将样品在模拟那些在房子里穿袜子的人的日常生活的条件下孵育：（1）90%湿度8小时和100%湿度16小时，以及那些在房子里赤脚的人：（2）80%湿度8小时和100%湿度16小时。每隔24小时取一次标本，洗涤前后进行PAS染色和扫描电镜观察。虽然真菌成分在（1）中不可去除，但即使在一天后清洗，它们在（2）中可以在一天和两天后去除。我们认为，即使皮肤真菌已经开始渗透角质层表面，真菌元素也很容易去除，因为当鞋子被脱掉时，鞋底保持低湿度，并且鞋底和趾间区域每天都要清洗。此外，在假设为真菌的实验中，将真菌成分施加到角质层的切割侧，在80%湿度下孵育，并在PAS染色后观察。断端毛癣菌在0.5天内开始穿透皮肤角质层，比其他皮肤癣菌更快。我们相信这是T.在日本的感染。
After several types of dermatophytes were applied to the stratum corneum obtained from a healthy human heel, we evaluated the penetration speed of fungal elements into the stratum corneum and the effect of washing its surface. We designed the following two environments, assuming tinea pedis after applying fungal elements to the surface of the stratum corneum. The samples were incubated under conditions simulating the daily life of those who wear socks in a house: (1) 90% humidity for 8 hours, and 100% humidity for 16 hours, and those who have bare feet in a house: (2) 80% humidity for 8 hours and 100% humidity for 16 hours. We took the samples out every 24 hours and made observations by PAS stain and scanning electron microscope before and after washing them. Although fungal elements were not removable in (1), even if washed one day later, they could be removable one and two days later in (2). We suggest that fungal elements were easily removable even if dermatophytes had begun to penetrate the surface of the stratum corneum, because the soles retained a low humidity when the shoes were removed, and the soles and interdigital regions were washed every day. Moreover, in an experiment assuming tinea corporis, fungal elements were applied to the cutting side of the stratum corneum, incubated at 80% humidity, and observed after PAS stain. The penetration of Trichophyton tonsurans to the cutting side of the stratum corneum began in 0.5 days, which was sooner than other dermatophytes. We believe this is one factor of the latest expansion of T. tonsurans infection in Japan.