Expression of trypsin by epithelial cells of various tissues, leukocytes, and neurons in human and mouse
Expression of trypsin by epithelial cells of various tissues, leukocytes, and neurons in human and mouse
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DOI：
10.1016/s0002-9440(10)65635-0
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发表时间：
1998-09-01
影响因子：
6
通讯作者：
Miyazaki, K
中科院分区：
文献类型：
--
作者：
Koshikawa, N;Hasegawa, S;Miyazaki, K
胰蛋白酶在正常情况下仅在胰腺中合成，本研究检测了胰蛋白酶在人和小鼠非胰腺组织中的表达，正常人组织的北方印迹分析表明胰蛋白酶基因在胰腺和脾脏中以高水平表达，在小肠中也有相当大的表达，然而，原位杂交和免疫组织化学证实胰蛋白酶广泛表达于皮肤、食管、胃、小肠、肺、肾、肝和肝外胆管的上皮细胞以及脾和神经元细胞。在脾脏中，在海马和大脑皮质的神经细胞中检测到胰蛋白酶信息，通过明胶酶谱分析证实在各种正常小鼠组织中存在潜在或活性形式的胰蛋白酶。逆转录-聚合酶链反应分析还证实了正常小鼠的脾脏、肝脏、肾脏和大脑中胰蛋白酶基因的表达。胰蛋白酶如此广泛的分布表明其在维持正常上皮细胞功能、免疫防御系统和中枢神经系统中的一般作用。
It has long been believed that trypsin is normally synthesized only in the pancreas, In the present study, expression of trypsin in human and mouse nonpancreatic tissues was examined, Northern blot analysis of normal human tissues indicated that the trypsin gene is expressed at high levels in the pancreas and spleen and considerably in the small intestine, However, in situ hybridization and immunohistochemistry demonstrated that trypsin is widely expressed in epithelial cells of the skin, esophagus, stomach, small intestine, lung, kidney, liver, and extrahepatic bile duct, as well as splenic and neuronal cells. In the spleen, trypsin message was detected in the nerve cells of the hippocampus and cerebral cortex, Analysis by gelatin zymography confirmed the presence of a latent or an active form of trypsin in various normal mouse tissues. Reverse transcription-polymerase chain reaction analysis also confirmed the expression of trypsin genes in the spleen, liver, kidney, and brain of normal mice. Such a broad distribution of trypsin suggests its general roles in the maintenance of normal epithelial cell functions, the immune defense system, and the central nervous system.