INHIBITORY EFFECT OF DEOXYNIVALENOL, 3-ACETYLDEOXYNIVALENOL AND ZEARALENONE ON INDUCTION OF RAT AND HUMAN-LYMPHOCYTE PROLIFERATION
INHIBITORY EFFECT OF DEOXYNIVALENOL, 3-ACETYLDEOXYNIVALENOL AND ZEARALENONE ON INDUCTION OF RAT AND HUMAN-LYMPHOCYTE PROLIFERATION
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DOI：
10.1016/0378-4274(84)90129-2
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发表时间：
1984-01-01
影响因子：
3.5
通讯作者：
MILLER, K
中科院分区：
文献类型：
--
作者：
ATKINSON, HAC;MILLER, K
用大鼠和人外周血淋巴细胞（PBL）体外培养，观察脱氧雪腐镰刀菌烯醇（DON）、3-乙酰脱氧雪腐镰刀菌烯醇（acetylDON）和玉米赤霉烯酮（Ze）对丝裂原诱导的淋巴细胞转化的影响。每种霉菌毒素均显示淋巴细胞增殖呈剂量依赖性降低。DON的抑制作用显著高于乙酰化化合物。90和220 ng/ml的浓度分别抑制大鼠和人淋巴细胞胚细胞生成50%，需要450和1060 ng/ml的乙酰DON产生相同的效果。抑制50%胚细胞生成所需的Ze量是DON所需量的250倍。没有细胞死亡的证据，DON和Ze的组合没有改变预期的反应。
Deoxynivalenol (DON), 3-acetyldeoxynivalenol (acetylDON) and zearalenone (Ze) were examined for their in vitro effect on mitogen-induced lymphocyte blastogenesis using rat or human peripheral blood lymphocytes (PBL). A dose-dependent reduction of lymphocyte proliferation was demonstrated for each mycotoxin. The inhibitory effect of DON was significantly higher than that of the acetylated compound. Concentrations of 90 and 220 ng/ml inhibited rat and human lymphocyte blastogenesis by 50%, respectively; 450 and 1060 ng/ml acetylDON were required to produce the same effect. The amount of Ze necessary to inhibit blastogenesis by 50% was 250-fold greater than required for DON. There was no evidence of cell death and combinations of DON and Ze did not alter the expected response.