Characterization of gelatin nanofibers electrospun using ethanol/formic acid/water as a solvent
Characterization of gelatin nanofibers electrospun using ethanol/formic acid/water as a solvent
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DOI：
10.1002/pat.1244
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发表时间：
2009-02-01
影响因子：
3.4
通讯作者：
Yang, Ming-Chien
中科院分区：
文献类型：
--
作者：
Chen, Hsin-Chieh;Jao, Win-Chun;Yang, Ming-Chien
以甲酸和乙醇水溶液代替细胞毒性溶剂，采用静电纺丝法制备明胶纳米纤维。进一步与戊二醛（GTA）交联。考察了贮存时间对溶液粘度和凝胶点的影响。利用场发射扫描电子显微镜（FESEM）对明胶纳米纤维的尺寸和形貌进行了研究。当明胶浓度为20 wt%，在常温下以20 kV的电压在10 cm的距离上进行静电纺丝时，明胶纤维的最小直径（85 nm，不含珠）达到。差示扫描量热法（DSC）结果表明，与GTA交联的明胶纳米纤维软化温度升高。此外，gta交联的明胶纳米纤维对小鼠系膜细胞具有细胞相容性（CRL 1927）。版权所有(C) 2008约翰威利父子有限公司
Gelatin nanofibers were prepared via electrospinning using aqueous solutions of formic acid and ethanol as the solvent instead of cytotoxic solvents. The resulting mat was further crosslinked with glutaraldehyde (GTA). The influence of the storing time on the viscosity and gel point of the solution was investigated. The gelatin nanofibers were examined using a field emission scanning electron microscope (FESEM) for the fiber size and morphology. The lowest diameter of gelatin fiber (85 nm, without beads) was achieved when the gelatin concentration was 20 wt% and electrospinning was conducted with a voltage of 20 kV over a distance of 10 cm at ambient temperature. The results from differential scanning calorimetry (DSC) showed that the softening temperature of gelatin nanofibers crosslinked with GTA was elevated. In addition, GTA-crosslinked gelatin nanofibers exhibited cell compatibility for mouse mesangial cells (CRL 1927). Copyright (C) 2008 John Wiley & Sons, Ltd.