Effects of high glucose plus high insulin on proliferation and apoptosis of mouse endothelial progenitor cells
Effects of high glucose plus high insulin on proliferation and apoptosis of mouse endothelial progenitor cells
复制标题
高糖联合高胰岛素对小鼠内皮祖细胞增殖和凋亡的影响
DOI：
10.1007/s00011-008-8125-3
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发表时间：
2008-11
影响因子：
6.7
通讯作者：
R.Hu
中科院分区：
文献类型：
--
作者：
S.Chen;W.Zhang;G.Zhang;H.Jin;R.Qian;X.Wang;R.Hu
目的研究高糖高胰岛素（GI）对内皮祖细胞（EPC）增殖、凋亡、形态及分化的影响。MTT法检测EPC增殖，Annexin-V/PI双标记法检测EPC凋亡。Western blotting.Results7天后GI处理，EPC细胞增殖和凋亡相关因素进行了检查，在G 0/G1期被逮捕。与对照组相比，高糖组或高胰岛素组EPC增殖及cyclin E、cdk 2和PCNA表达均降低。HG对EPC增殖和生长相关蛋白的抑制作用明显强于GI。高糖、GI和HI均沿着caspase-3的过度表达，促进EPC凋亡。结论GI诱导的EPC凋亡和生长抑制可能是糖尿病EPC减少和功能障碍的机制之一。
ObjectiveTo study the effects of high glucose plus high insulin (GI) on proliferation, apoptosis, morphology and differentiation of endothelial progenitor cells (EPC).MethodsEPC were isolated and identified by magnetic cell sorting and flow cytometry. EPC proliferation and apoptosis were measured by MTT assay and Annexin-V/PI double labeling, respectively. Cell proliferation- and apoptosis-related factors were examined by Western blotting.ResultsSeven days after GI treatment, EPC were arrested at the G0/G1phase. Treatment with high glucose alone (HG) or GI but not HI (high insulin alone) decreased EPC proliferation and their expression of cyclin E, cdk2 and PCNA compared to control. The inhibitory effects of HG on EPC proliferation and growth-related proteins were more significant than those of GI. HG, GI and HI promoted EPC apoptosis along with caspase-3 overexpression.ConclusionThe result indicated that GI-induced apoptosis and growth inhibition might be one of mechanisms of EPC reduction and dysfunction in diabetes.
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影响因子：
4.8
作者：
V. Yadav;G. Lakshmi;R. Medhamurthy
通讯作者：
V. Yadav;G. Lakshmi;R. Medhamurthy
影响因子：
25.7
作者：
Lena Koroxenidou;L. Ohlson;I. Porsch Hällström
通讯作者：
Lena Koroxenidou;L. Ohlson;I. Porsch Hällström
DOI：
10.1083/jcb.127.2.537
发表时间：
1994-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Re F;Zanetti A;Sironi M;Polentarutti N;Lanfrancone L;Dejana E;Colotta F
通讯作者：
Colotta F
影响因子：
8.2
作者：
Wang, Ming-xia;Ren, Lei-ming
通讯作者：
Ren, Lei-ming
影响因子：
--
作者：
S. McAllister;A. Moses
通讯作者：
S. McAllister;A. Moses