Cis-acting sequences responsible for the transcriptional activation of human T-cell leukemia virus type I constitute a conditional enhancer.
Cis-acting sequences responsible for the transcriptional activation of human T-cell leukemia virus type I constitute a conditional enhancer.
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负责人 T 细胞白血病病毒 I 型转录激活的顺式作用序列构成了条件增强子。
DOI：
10.1073/pnas.83.17.6558
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发表时间：
1986
影响因子：
11.1
通讯作者：
Pavlakis,GN
中科院分区：
文献类型：
--
作者：
Paskalis,H;Felber,BK;Pavlakis,GN
人T细胞白血病病毒I型的长末端重复启动子的转录在基因组3'部分编码的反式激活蛋白TA-1存在下被激活。已经在瞬时表达测定中研究了一系列长末端重复突变体和杂合启动子构建体在反式激活蛋白存在下被激活的能力。负责反式激活的序列具有与转录增强子元件相似的性质。它们的作用相对独立于位置和方向，并且仅在反式激活蛋白存在下激活同源和异源启动子。因此，人T细胞白血病病毒I型的反式激活蛋白通过诱导增强机制起作用。
Transcription from the long terminal repeat promoter of human T-cell leukemia virus type I is activated in the presence of a trans-activator protein, TA-I, encoded in the 3' part of the genome. A series of long terminal repeat mutants and hybrid promoter constructs have been studied in a transient expression assay for their ability to be activated in the presence of the trans-activator protein. The sequences responsible for trans-activation have properties similar to those of transcription enhancer elements. They act relatively independent of position and orientation and activate both the homologous as well as heterologous promoters only in the presence of the trans-activator protein. Therefore, the trans-activator protein of human T-cell leukemia virus type I acts via an inducible enhancement mechanism.