Age-dependent response to insecticides and enzymatic variation in susceptible and resistant codling moth larvae
Age-dependent response to insecticides and enzymatic variation in susceptible and resistant codling moth larvae
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DOI：
10.1002/arch.10052
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发表时间：
2002-10-01
影响因子：
2.2
通讯作者：
Sauphanor, B
中科院分区：
文献类型：
--
作者：
Bouvier, JC;Boivin, T;Sauphanor, B
苹果蠹蛾（Cydia pomonello）的杀虫剂抗性部分是由于代谢解毒能力增强所致。本研究的目的是追踪一种易感品系（S）和两种抗性品系（Rv 和 Rt）中幼虫对溴氰菊酯和氟苯脲敏感性的年龄变化，分别选择对溴氰菊酯和除虫脲具有抗性的品系。同时研究了 S 菌株和两种抗性菌株中细胞色素 P450 依赖性单加氧酶 (MFO)、谷胱甘肽 S 转移酶 (GST) 和酯酶活性的年龄变化。尽管 Rv 新生儿对溴氰菊酯表现出增强的抗性，但两种抗性品系在晚期都记录了最高水平的杀虫剂抗性。先前在 Rv 菌株中的 kdr 点突变的证明支持了额外的溴氰菊酯特异性抗性机制的参与，该机制主要在早期龄期中表达。溴氰菊酯和氟苯脲之间的交叉耐药性表明非特异性代谢途径参与了氟苯脲耐药性，而不是靶位点修饰。 GST 活性增强与溴氰菊酯抗性之间的正相关表明，这种机制可能发生在苹果蠹蛾对拟除虫菊酯处理的适应性反应中。与敏感菌株相比，两种抗性菌株的每一龄都记录到增强的 MFO 活性。但这些活性与对溴氰菊酯和氟苯脲的反应均不相关。根据这些发现，研究选择反应的年龄依赖性对于实施阻力监测测试至关重要，特别是在难以在现场收集目标安装的情况下。拱。 (C) 2002 Wiley-Liss, Inc.
Insecticide resistance in the codling moth, Cydia pomonello, partly results from increased metabolic detoxification, The aim of this study was to follow the age variations in larval susceptibility to deltamethrin and teflubenzuron in one susceptible (S) strain, and two resistant (Rv and Rt) ones selected for resistance to deltamethrin and diflubenzuron, respectively. The age variation of the activities of cytochrome P450-dependent monooxygenase (MFO), glutathione S-transferases (GST), and esterases in S and both resistant strains were simultaneously investigated. The highest levels of insecticide resistance were recorded in late instars in both resistant strains, although Rv neonates exhibited enhanced resistance to deltamethrin. The involvement of an additional deltamethrin-specific mechanism of resistance, which could be mainly expressed in early instars, was supported by previous demonstration of a kdr point mutation in the Rv strain. The cross-resistance between deltamethrin and teflubenzuron indicated the involvement of non-specific metabolic pathways in resistance to teflubenzuron, rather than target site modification. A positive correlation between enhanced GST activities and deltamethrin resistance suggested that this mechanism might take place into the adaptive response of C. pomonella to pyrethroids treatments. Enhanced MFO activity was recorded in each instar of the two resistant strains compared to the susceptible one. But these activities were not correlated to the responses to deltamethrin nor to teflubenzuron. In the light of these findings, studying age-dependence of responses to selection is central to the implementation of monitoring tests of resistances, especially if the target installs are difficult to collect in the field. Arch. (C) 2002 Wiley-Liss, Inc.