Platelet-rich plasma as an additional therapeutic option for infected wounds with multi-drug resistant bacteria: in vitro antibacterial activity study
Platelet-rich plasma as an additional therapeutic option for infected wounds with multi-drug resistant bacteria: in vitro antibacterial activity study
复制标题
DOI：
10.1007/s00068-018-0957-0
复制
发表时间：
2019-06-01
影响因子：
2.1
通讯作者：
Unlu, Aytekin
中科院分区：
文献类型：
--
作者：
Cetinkaya, Riza Aytac;Yenilmez, Ercan;Unlu, Aytekin
目的感染伤口，如糖尿病足感染，大多是多种微生物，微生物对抗菌药物具有很高的耐药率。糖尿病患者感染伤口的费用、发病率和死亡率都很高。基于这些事实，需要支持性局部治疗方案，例如实施富血小板血浆（PRP）。我们的目标是展示体外抗菌效果，以开展针对糖尿病足感染等感染伤口局部 PRP 实施的临床试验。在本研究中，我们旨在证明 PRP 对耐甲氧西林金黄色葡萄球菌 (MRSA) 和另外三种在伤口感染中重要且难以治疗的多重耐药细菌的体外抗菌活性。材料和方法自体 PRP、贫血小板血浆 (PPP) 和磷酸盐缓冲盐水 (PBS) 对耐甲氧西林金黄色葡萄球菌的体外抗菌活性，通过评估琼脂平板上的细菌生长情况和药敏试验结果，比较耐万古霉素肠球菌、产广谱β内酰胺酶肺炎克雷伯菌和耐碳青霉烯类铜绿假单胞菌。结果与对照组相比，PRP和PPP在第1、2、5和5天显着抑制MRSA、肺炎克雷伯菌和铜绿假单胞菌的细菌生长。孵育第 10 小时（p
PurposeInfected wounds, such as diabetic foot infections, are mostly polymicrobial and microorganisms have high resistance rates to antimicrobials. Infected wounds in diabetic patients have high cost, morbidity, and mortality rates. Based on these facts, there is a need for supportive localized treatment options such as platelet-rich plasma (PRP) implementations. Demonstrating the in vitro antimicrobial effect, our aim was to lead up to clinical trials of localized PRP implementations in infected wounds such as diabetic foot infections. In this study, we aimed to demonstrate the in vitro antibacterial activity of PRP against methicilin-resistant Staphylococcus aureus (MRSA) and three more multi-drug resistant bacteria species that are important and hard-to-treat in wound infections.Materials and methodsIn vitro antimicrobial activity of autologous PRP, platelet-poor plasma (PPP), and phosphate-buffered saline (PBS) on methicillin-resistant Staphylococcus aureus, vancomycin-resistant Enterococcus spp., extended spectrum beta lactamase producing Klebsiella pneumoniae, and carbapenem-resistant Pseudomonas aeruginosa was compared by assessment of bacterial growth on agar plates and antimicrobial susceptibility test results.ResultsWhen compared to control group, PRP and PPP significantly suppressed bacterial growth of MRSA, K. pneumoniae, and P. aeruginosa at 1st, 2nd, 5th, and 10th hours of incubation (p