Transcriptome dynamics of Arabidopsis during sequential biotic and abiotic stresses
Transcriptome dynamics of Arabidopsis during sequential biotic and abiotic stresses
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DOI：
10.1111/tpj.13167
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发表时间：
2016-05-01
期刊：
影响因子：
7.2
通讯作者：
Van Wees, Saskia C. M.
中科院分区：
文献类型：
--
作者：
Coolen, Silvia;Proietti, Silvia;Van Wees, Saskia C. M.
在自然界中，植物必须应对经常同时或依次发生的各种胁迫条件。为了研究植物如何应对多重胁迫条件，我们分析了拟南芥暴露于六种连续双重胁迫下的动态，这些胁迫由以下组合造成：（i）坏死营养真菌灰葡萄孢感染，（ii）咀嚼菜青虫幼虫的食草性，以及（iii）干旱胁迫。随着时间的推移，这些胁迫中的每一个都会诱导特定的表达谱，其中所有差异表达基因的三分之一由至少两个单一胁迫共享。其中，394 个基因在所有三种应激条件下都有差异表达，尽管通常方向相反。当依次施加两种胁迫时，无论第一种胁迫的性质如何，植物都表现出与第二种胁迫非常相似的转录组谱。然而，可以在连续应力分布中识别出重要的第一应力特征。对共表达基因簇动态的生物信息分析突出了特定簇和生物过程，其中激活或抑制的时间因先前的应激而改变。第二应激转录谱中的第一应激特征通常与对植物激素的反应相关，这强化了激素是不同类型应激之间相互作用的全局调节剂的概念。由于先前的应激会影响对后续应激的耐受水平（例如，先前的食草性强烈影响对灰霉病菌的抵抗力），因此首次应激特征可以为识别在应激反应途径之间的相互作用中起决定性作用的分子参与者提供重要线索。
In nature, plants have to cope with a wide range of stress conditions that often occur simultaneously or in sequence. To investigate how plants cope with multi-stress conditions, we analyzed the dynamics of whole-transcriptome profiles of Arabidopsis thaliana exposed to six sequential double stresses inflicted by combinations of: (i) infection by the necrotrophic fungus Botrytis cinerea, (ii) herbivory by chewing larvae of Pieris rapae, and (iii) drought stress. Each of these stresses induced specific expression profiles over time, in which one-third of all differentially expressed genes was shared by at least two single stresses. Of these, 394 genes were differentially expressed during all three stress conditions, albeit often in opposite directions. When two stresses were applied in sequence, plants displayed transcriptome profiles that were very similar to the second stress, irrespective of the nature of the first stress. Nevertheless, significant first-stress signatures could be identified in the sequential stress profiles. Bioinformatic analysis of the dynamics of co-expressed gene clusters highlighted specific clusters and biological processes of which the timing of activation or repression was altered by a prior stress. The first-stress signatures in second stress transcriptional profiles were remarkably often related to responses to phytohormones, strengthening the notion that hormones are global modulators of interactions between different types of stress. Because prior stresses can affect the level of tolerance against a subsequent stress (e.g. prior herbivory strongly affected resistance to B. cinerea), the first-stress signatures can provide important leads for the identification of molecular players that are decisive in the interactions between stress response pathways.