TRUNCATED FORMS OF HUMAN-COMPLEMENT FACTOR-H
TRUNCATED FORMS OF HUMAN-COMPLEMENT FACTOR-H
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DOI：
10.1042/bj2580927
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发表时间：
1989-03-15
影响因子：
4.1
通讯作者：
RIPOCHE, J
中科院分区：
文献类型：
--
作者：
FONTAINE, M;DEMARES, MJ;RIPOCHE, J
通过使用多克隆抗（H因子）抗体的蛋白质印迹分析，在非还原条件下Mr 41 000和37 000以及还原条件下Mr 43 000和40 000的两种低Mr蛋白质种类与人血浆和血清中众所周知的155 000-Mr H因子一起被一致地检测到。这两种其他物质也存在于血浆、尿液和滑液中。41 000-Mr物质，而不是37 000-Mr物质，但不是41 000-Mr物质，可通过针对H因子C-末端部分的单克隆抗（H因子）抗体检测到。41 000-Mr和37 000-Mr物质与已充分表征的36 000-Mr H因子N-末端胰蛋白酶片段不同。它们可能代表我们最近描述的人类肝脏中存在的1.8 kb和1.2-1.5 kb短因子H mRNA种类的翻译产物。
By the use of Western-blot analyses with polyclonal anti-(Factor H) antibodies, two low-Mr protein species of Mr 41 000 and 37 000 under non-reducing conditions and 43 000 and 40 000 under reducing conditions are consistently detected together with the well-known 155 000-Mr Factor H in human plasma and serum. These two additional species are also found in plasma, urine and synovial fluids. The 41 000-Mr species but not the 37 000-Mr species but not the 41 000-Mr species is detected by a monoclonal anti-(Factor H) antibody directed at the C-terminal part of Factor H. The 41 000-Mr and 37 000-Mr species are different from the well-characterized 36 000-Mr N-terminal tryptic fragment of Factor H. They are likely to represent translational products of the short Factor H mRNA species of 1.8 kb and 1.2-1.5 kb occurring in human liver that we have recently described.