Singleton Sequence Type 382, an Emerging Clonal Group of Listeria monocytogenes Associated with Three Multistate Outbreaks Linked to Contaminated Stone Fruit, Caramel Apples, and Leafy Green Salad.
Singleton Sequence Type 382, an Emerging Clonal Group of Listeria monocytogenes Associated with Three Multistate Outbreaks Linked to Contaminated Stone Fruit, Caramel Apples, and Leafy Green Salad.
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DOI：
10.1128/jcm.02140-16
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发表时间：
2017-03
影响因子：
9.4
通讯作者：
Strain EA
中科院分区：
文献类型：
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作者：
Chen Y;Luo Y;Pettengill J;Timme R;Melka D;Doyle M;Jackson A;Parish M;Hammack TS;Allard MW;Brown EW;Strain EA
2014年至2016年期间，涉及美国国内外病例患者的三次多州疫情与核果、焦糖苹果和包装的多叶绿色沙拉有关，这些蔬菜被单核细胞增生李斯特菌单细胞序列型382（ST 382）污染，这是一种基因组差异有限的血清型IVb-v1克隆。通过全基因组测序（WGS）分析对来自这些暴发的分离株和与这些暴发无关的其他ST 382分离株进行了分析。ST 382菌株之间的主要差异是单核苷酸多态性（SNP）。WGS分析将ST 382与共污染焦糖苹果的克隆复合物1爆发菌株区分开。WGS在每次爆发中聚集了食物、环境和临床分离株，并区分了三种爆发菌株和流行病学上不相关的ST 382分离株，这些分离株通过脉冲场凝胶电泳无法区分。ST 382似乎是一个新兴的克隆，在2016年前大约32年开始与其祖先分离。我们估计，有1.29个核苷酸替换每个基因组（2.94 Mbp）每年为该克隆。
Three multistate outbreaks between 2014 and 2016, involving case patients in and outside the United States, were linked to stone fruit, caramel apples, and packaged leafy green salad contaminated with Listeria monocytogenes singleton sequence type 382 (ST382), a serotype IVb-v1 clone with limited genomic divergence. Isolates from these outbreaks and other ST382 isolates not associated with these outbreaks were analyzed by whole-genome sequencing (WGS) analysis. The primary differences among ST382 strains were single nucleotide polymorphisms (SNPs). WGS analysis differentiated ST382 from a clonal complex 1 outbreak strain co-contaminating the caramel apples. WGS clustered food, environmental, and clinical isolates within each outbreak, and also differentiated among the three outbreak strains and epidemiologically unrelated ST382 isolates, which were indistinguishable by pulsed-field gel electrophoresis. ST382 appeared to be an emerging clone that began to diverge from its ancestor approximately 32 years before 2016. We estimated that there was 1.29 nucleotide substitution per genome (2.94 Mbp) per year for this clone.