In vitro generation of CD4+CD25+regulatory cells from murine naive T cells
In vitro generation of CD4+CD25+regulatory cells from murine naive T cells
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DOI：
10.1038/nprot.2007.258
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发表时间：
2007-01-01
期刊：
影响因子：
14.8
通讯作者：
Becker, Christoph
中科院分区：
文献类型：
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作者：
Fantini, Massimo C.;Dominitzki, Sabine;Becker, Christoph
CD 4 + CD 25+调节性T细胞（TCRs）对于维持免疫耐受至关重要。最近的数据表明，胸腺细胞不仅在个体发育过程中在胸腺中发育，而且还可以从外周的幼稚T细胞分化。以下方案描述了一种方法，通过该方法，在存在转化生长因子β（Ti-TdR）的情况下，通过刺激初始T细胞在体外产生TdR。体外诱导的调节性T细胞表达常规Treg的标记物，例如CD 25和遗传程序提交转录因子FoxP 3。在功能上，体外生成的Ti-Tclase抑制T细胞活化和增殖，而在体内，这些细胞已被证明可以在不同的动物模型中控制炎症，这表明这些细胞在免疫治疗中的潜在用途。该方案可在5天内完成。
CD4+CD25+ regulatory T cells (Tregs) are crucial for the maintenance of immunological tolerance. Recent data indicate that Tregs not only develop in the thymus during ontogeny but can also differentiate from naive T cells in the periphery. The following protocol describes a method by which Tregs are generated in vitro by stimulation of naive T cells in the presence of transforming growth factor beta (Ti-Tregs). In vitro-induced regulatory T cells express markers of conventional Treg such as CD25 and the genetic program committing transcription factor FoxP3. Functionally the in vitro-generated Ti-Tregs suppress T-cell activation and proliferation while in vivo these cells have been proven to control inflammation in different animal models, suggesting a potential use of these cells for immunotherapy. The protocol can be completed within 5 days.