Comparison of the effects of transforming growth factor beta, N,N-dimethylformamide, and retinoic acid on transformed and nontransformed fibroblasts.
Comparison of the effects of transforming growth factor beta, N,N-dimethylformamide, and retinoic acid on transformed and nontransformed fibroblasts.
复制标题
比较转化生长因子β、N,N-二甲基甲酰胺和视黄酸对转化和非转化成纤维细胞的影响。
DOI：
10.1016/0014-4827(88)90260-1
复制
发表时间：
1988
影响因子：
3.7
通讯作者：
Brattain,MG
中科院分区：
文献类型：
--
作者：
Hoosein,NM;Brattain,DE;McKnight,MK;Brattain,MG
为了比较转化生长因子（TGFβ）与分化促进剂N，N-二甲基甲酰胺（DMF）和维甲酸（RA）的作用，检测了这三种物质的抗增殖和纤连蛋白诱导活性。使用AKR-2B小鼠胚胎成纤维细胞及其化学转化的对应物AKR-MCA细胞作为模型系统。TGFβ（EC 50 ~1 ng/ml）、DMF（EC 50 ~0.5%）和RA（EC 50 ~1 μM）对两种细胞系的单层生长均有抑制作用，且呈浓度依赖性。在所有三种药物中也观察到纤维连接蛋白表达的时间依赖性升高。TGFβ抑制两种细胞生长的EC_（50）与刺激纤维连接蛋白合成的EC_（50）一致。在AKR-2B细胞中暴露于TGFβ 3小时足以获得在48小时观察到的最大纤连蛋白水平，但在AKR-MCA细胞中没有。我们的结果表明，在该系统中，TGFβ的作用类似于化学分化诱导剂DMF和RA。此外，我们的数据还表明，转化和未转化的成纤维细胞对TGFβ信号的处理可能不同。
In order to compare the effects of transforming growth factor (TGFβ) with those of the differentiation promotersN,N-dimethylformamide (DMF) and retinoic acid (RA), the antiproliferative and fibronectin-inducing activities of the three agents were examined. AKR-2B mouse embryo fibroblasts and their chemically transformed counterpart AKR-MCA cells were used as the model system. Growth in monolayer culture of both cell lines was inhibited by TGFβ (EC50~1 ng/ml), DMF (EC50~0.5%), and RA (EC50~1 μM) in a concentration-dependent manner. Time-dependent elevation in fibronectin expression was also observed with all three agents. The EC50for growth inhibition of both cell lines by TGFβ agreed well with that obtained for stimulation of fibronectin synthesis. A 3-h exposure to TGFβ is sufficient to obtain the maximal fibronectin level observed at 48 h in AKR-2B cells but not in AKR-MCA cells. Our results indicate that in this system the effects of TGFβ are similar to those of the chemical differentiation inducers DMF and RA. Furthermore, our data also suggest that the TGFβ signal may be processed differently by nontransformed and transformed fibroblasts.