Effects of neonatal freeze lesions on expression of parvalbumin in rat neocortex.
Effects of neonatal freeze lesions on expression of parvalbumin in rat neocortex.
复制标题
新生儿冷冻损伤对大鼠新皮质小白蛋白表达的影响。
DOI：
10.1093/cercor/8.8.753
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Prince,DA
中科院分区：
文献类型：
--
作者：
Rosen,GD;Jacobs,KM;Prince,DA
新生儿皮质板冻结病变导致大脑皮质局灶性畸形，类似于四层小脑回。这些畸形与神经元结构的局部和远处改变有关，并与新皮质癫痫样放电有关，这种放电可以扩散到离畸形本身4毫米远的地方。为了评估这种畸形中一种抑制性中间神经元群体发育的潜在变化，我们在出生后第13、15、21和64天测量了小脑回和控制大脑皮层中的ParvIR（小蛋白免疫反应）神经元的密度。与对照组相比，微回动物在畸形本身和与其相邻2毫米的正常六层皮层中表现出小白蛋白免疫反应性表达的短暂性降低。这种差异在P21时消失。此外，小回内和紧挨着小回的颗粒内层的ParvIR神经元密度永久性降低。这些结果表明，早期皮质板损伤会引起局部和更广泛的皮质结构改变。
Neonatal freeze lesions to the cortical plate result in focal malformations of the cerebral cortex that resemble four-layered microgyria. These malformations have been associated with local and distant changes in neuronal architecture, and have been implicated in the neocortical epileptiform discharges that can spread up to 4 mm away from the malformation itself. In an effort to assess potential changes in the development of one population of inhibitory interneurons in this malformation, we measured the density of parvalbumin-immunoreactive (ParvIR) neurons in microgyric and control cerebral cortex on postnatal days 13, 15, 21 and 64. In comparison to controls, microgyric animals exhibited a transient decrease in the expression of parvalbumin immunoreactivity in supragranular neurons, both within the malformation itself and in normal six-layered cortex up to 2 mm adjacent to it. This difference disappeared by P21. In addition, there was a permanent diminution of the density of ParvIR neurons in infragranular layers both within and immediately adjacent to the microgyrus. These results indicate that early injury to the cortical plate gives rise to both focal and more widespread changes in cortical architecture.