Cutting edge:: CD8+CD122+ regulatory T cells produce IL-10 to suppress IFN-γ production and proliferation of CD8+ T cells
Cutting edge:: CD8+CD122+ regulatory T cells produce IL-10 to suppress IFN-γ production and proliferation of CD8+ T cells
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DOI：
10.4049/jimmunol.175.11.7093
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发表时间：
2005-12-01
影响因子：
4.4
通讯作者：
Suzuki, H
中科院分区：
文献类型：
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作者：
Endharti, AT;Rifa'I, M;Suzuki, H
我们最近鉴定了CD 8（+）CD 122（+）调节性T细胞，其直接控制CD 8（+）和CD 4（+）细胞而无需APC的干预。在这项研究中，我们研究了CD 8 + CD 122+调节性T细胞的效应机制，通过使用体外调节系统。细胞因子表达谱显示IL-10主要由CD 8（+）CD 122（+）细胞产生，而其他细胞因子在CD 8 + CD 122+细胞和CD 8（+）CD 122（-）细胞中类似地表达。通过向培养物中加入抗IL-10 Ab而不是加入抗TGF-β Ab，可以阻断CD 8、CD 8（+）CD 122（+）细胞对CD 122-细胞增殖和IFN-γ产生的抑制。当从CD 8（+）CD 122（+）细胞的条件培养基中去除IL-10时，条件培养基不再显示调节活性。最后，来自IL-10缺陷小鼠的CD 8（+）CD 122（+）细胞在体外没有调节活性，在体内调节活性降低。我们的结果清楚地表明，IL-10是由CD 8（+）CD 122（+）细胞产生的，并介导这些细胞的调节活性。
We recently identified CD8(+) CD122(+) regulatory T cells that directly control CD8(+) and CD4(+) cells without intervention of APCs. In this study, we investigated the effector mechanism of CD8+ CD122+ regulatory T cells by using an in vitro regulation system. The profile of cytokine expression revealed that IL-10 was predominantly produced by CD8(+) CD122(+) cells, whereas other cytokines were similarly expressed in CD8+ CD122+ cells and CD8(+) CD122(-) cells. Suppression of both proliferation CD122- cells by and IFN-gamma production by CD8, CD8(+) CD122(+) cells was blocked by adding anti-IL-10 Ab to the culture but not by adding anti-TGF-beta Ab. When IL-10 was removed from the conditioned medium from CD8(+) CD122(+) cells, the conditioned medium no longer showed regulatory activity. Finally, CD8(+) CD122(+) cells from IL-10-deficient mice had no regulatory activity in vitro and reduced regulatory activity in vivo. Our results clearly indicate that IL-10 is produced by CD8(+) CD122(+) cells and mediates the regulatory activity of these cells.