Time-dependent alterations in ethanol intake in male wistar rats exposed to short and prolonged daily maternal separation in a 4-bottle free-choice paradigm
Time-dependent alterations in ethanol intake in male wistar rats exposed to short and prolonged daily maternal separation in a 4-bottle free-choice paradigm
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DOI：
10.1111/j.1530-0277.2006.00247.x
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发表时间：
2006-12-01
影响因子：
3.2
通讯作者：
Nylander, Ingrid
中科院分区：
文献类型：
--
作者：
Gustafsson, Lisa;Nylander, Ingrid
背景资料：先前的研究表明，母体分离可以用于早期环境对成人乙醇摄入量影响的动物研究。这些研究表明，短期的每日分离导致低乙醇摄入量，而长期分离与高乙醇摄入量的风险增加有关。本研究的目的是进一步研究的长期影响的早期生活事件对乙醇insufficient.Methods：幼鼠暴露于15分钟（MS 15）或360分钟（MS 360）的日常母亲分离在出生后的第1天至21天或保持在正常的动物设施饲养（AFR）条件下。在成年期，雄性大鼠自由获得5，10，和20%的乙醇，除了水，在4瓶的选择paradig.Results：没有差异，总的乙醇摄入量或偏好之间的3个实验groups. The 54天的饮酒期分为收购，稳定，和维护阶段的时间和组间差异的分析。随着时间的推移，MS 15大鼠的乙醇摄入量增加;在整个实验过程中，它们主要摄入5%的乙醇，并且摄入20%的乙醇。MS 360大鼠增加了乙醇摄入量，将偏好从5%改变为20%乙醇，并且随着时间的推移，20%乙醇的摄入量增加更高。结论：MS 15和MS 360大鼠对低浓度和高浓度乙醇的选择具有时间依赖性。结果支持以前的研究结果表明，MS 15可以作为一个模型的环境保护因素和MS 360代表了一个风险环境收购高成人乙醇摄入量。
Background: Previous studies have shown that maternal separation can be used in animal studies of early environmental influence on adult ethanol intake. These studies have shown that short daily separations result in low ethanol intake, whereas prolonged separations relate to an enhanced risk for a high ethanol intake. The aim of the present study was to further examine the long-term effects of early-life events on ethanol intake.Methods: Rat pups were exposed to 15 minutes (MS15) or 360 minutes (MS360) of daily maternal separation during postnatal days 1 to 21 or kept under normal animal facility rearing (AFR) conditions. In adulthood, male rats were given free access to 5, 10, and 20% ethanol, in addition to water, in a 4-bottle-choice paradigm.Results: No differences in total ethanol intake or preference between the 3 experimental groups were found. The 54-day drinking period was divided into acquisition, stabilization, and maintenance phases for analysis of time and group differences. The MS15 rats increased ethanol intake over time; they mostly consumed 5% ethanol and had a low intake of 20% ethanol throughout the experiment. MS360 rats increased ethanol intake, changed preference from 5% to 20% ethanol, and had a higher increase in intake of 20% ethanol over time. The ethanol intake and preference in the AFR rats were more similar to that of the MS360 rats.Conclusions: Time-dependent changes were observed in the preferred choice of low versus high ethanol concentrations in MS15 and MS360 rats. The results support previous findings suggesting that MS15 can be used as a model for environmental protective factors and that MS360 represents a risk environment for acquisition of a high adult ethanol intake.