Effects of different factors on the forward extraction of soy protein in reverse micelle systems
Effects of different factors on the forward extraction of soy protein in reverse micelle systems
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DOI：
10.5897/ajb12.202
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发表时间：
2012-04
影响因子：
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通讯作者：
Guanhao Bu;Hai-ying Liu;Fusheng Chen;Kunlun Liu;Yingying Yang;Yanxiu Gao
中科院分区：
文献类型：
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作者：
Guanhao Bu;Hai-ying Liu;Fusheng Chen;Kunlun Liu;Yingying Yang;Yanxiu Gao
反胶束萃取是一种新的蛋白质提取技术。本研究采用阴离子表面活性剂双（2-乙基己基）琥珀酸磺基钠（AOT）反胶束体系、十二烷基硫酸钠（SDS）反胶束体系和阳离子表面活性剂十六烷基三甲基溴化铵（CTAB）反胶束体系分别对大豆蛋白进行萃取。考察了豆粉浓度、Wo （[h2o]/[AOT]）、温度、时间、pH、离子强度和超声功率对大豆蛋白正向提取效率的影响。还研究了AOT反胶束直径的影响。AOT反胶束体系的萃取效率高于SDS和CTAB体系。影响正向提取的主要因素有豆粉浓度、温度和pH， AOT体系的最佳提取条件为豆粉浓度0.007 g/ml、w16、pH 6.5、温度34℃、时间20 min、KCl浓度0.1 mol/L、超声功率240 W。在此条件下，大豆蛋白的提取率为85.5%。AOT反胶束体系对大豆蛋白的正向萃取效率随着反胶束直径的增大而增大。反胶束萃取是一种有效的大豆蛋白提取方法。关键词：大豆蛋白，反胶束，正萃取，二（2-乙基己基）琥珀酸磺基钠（AOT），十二烷基硫酸钠（SDS），十六烷基三甲基溴化铵（CTAB）
Reverse micelle extraction is a new technology for the extraction of protein. In this research, three kinds of reverse micelle systems, anionic surfactant sodium bis(2-ethylhexyl) sulfosuccinate (AOT) reverse micelle system, sodium dodecyl sulfate (SDS) reverse micelle system, and cationic surfactant cetyltrimethyl ammonium bromide (CTAB) reverse micelle system, were used to extract soy protein respectively. Effects of soy flour concentration, Wo ([H 2 O]/[AOT]), temperature, time, pH, ionic strength and ultrasonic power on forward extraction efficiency of soy protein were investigated. The effect of AOT reverse micelle diameter was studied as well. AOT reverse micelle system had higher extraction efficiency than SDS and CTAB systems. The main factors that affected the forward extraction were soy flour concentration, temperature and pH. The optimal conditions in AOT system were soy flour concentration being 0.007 g/ml, Wo 16, pH 6.5, temperature 34°C, time 20 min, KCl concentration of 0.1 mol/L and ultrasound power of 240 W. Under these conditions, the extraction efficiency of soy protein was 85.5%. The forward extraction efficiency of soy protein in AOT reverse micelle system increased with the increase of the reverse micelle diameter. Reverse micelle extraction is an effective way to extract soy protein. Key words: Soy protein, reverse micelle, forward extraction, sodium bis(2-ethylhexyl) sulfosuccinate (AOT), sodium dodecyl sulfate (SDS), cetyltrimethyl ammonium bromide (CTAB).