Differential Changes of Local Blood Supply in Rat Retinae Are Involved in the Selective Loss of Retinal Ganglion Cells Following the Acute High Intraocular Pressure
Differential Changes of Local Blood Supply in Rat Retinae Are Involved in the Selective Loss of Retinal Ganglion Cells Following the Acute High Intraocular Pressure
复制标题
急性高眼压后大鼠视网膜局部血供的差异性变化与视网膜神经节细胞的选择性丢失有关
DOI：
10.3109/02713680903514675
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发表时间：
2010-05-01
影响因子：
2
通讯作者：
Luo, Xue-gang
中科院分区：
文献类型：
--
作者：
Tong, Jian-bin;Chen, Dan;Luo, Xue-gang
目的：检测急性高眼压后视网膜中央、中部和周围的局部血供变化，探讨视网膜局部血供变化对视网膜神经节细胞选择性损失的影响。方法：采用生理盐水灌注系统，将前房压升高至110 mmHg，建立Sprague-Dawley大鼠急性高眼压模型。采用明胶墨水灌注和荧光微球注射检测急性高眼压诱导后3、6、12 h和1、3、7、14 d中央、中、外周视网膜的血供情况。用氟金逆行标记法检测急性高眼压后视网膜神经节细胞丢失。最后，研究了视网膜局部血供变化与视网膜神经节细胞损失的关系。结果：急性高眼压后3 h ~ 14 d，周围视网膜血供增加比例低于中央和中间视网膜。在急性高眼压诱导后的前3 d内，周围视网膜神经节细胞损失的百分比明显大于中央和中间视网膜（p < 0.05）。无论是明胶墨水输注法还是微球注射法（p < 0.05），急性高眼压诱导后视网膜神经节细胞损失百分比与相应的局部血供增加呈显著负相关（r = -0.57或-0.72）。此外，外周视网膜呈显著负相关（r = -0.80或-0.91;p < 0.05）。结论：大鼠急性高眼压诱导后，视网膜中央、中部和周围视网膜局部血供变化存在差异。大鼠视网膜局部血供变化的不均匀性与视网膜神经节细胞的选择性丧失有关。
Purpose: To detect changes in local blood supply to central, middle, and peripheral retina following acute high intraocular pressure and to investigate the effects of changes in local retinal blood supply on the selective loss of retinal ganglion cells. Methods: The acute high intraocular pressure model of Sprague-Dawley rats was established by increasing the anterior chamber pressure to 110 mmHg via a normal saline perfusion system. Blood supply to the central, middle, and peripheral retina at 3, 6, and 12 h, and 1, 3, 7, and 14 d following induction of acute high intraocular pressure was detected by using gelatin-ink perfusion and fluorescent microsphere injection. Retinal ganglion cell loss following acute high intraocular pressure was detected by fluorogold retrograde labeling. Finally, the relationship between changes in local retinal blood supply and loss of retinal ganglion cells was investigated. Results: The increased ratio of blood supply of peripheral retina was less than that of the central and middle retina at 3 h to 14 d following acute high intraocular pressure. The percent of retinal ganglion cell loss in the peripheral retina was clearly greater than that in the central and middle retina during the first 3 d following induction of acute high intraocular pressure (p < 0.05). Using either the gelatin-ink infusion method or the microsphere injection method (p < 0.05), a significantly negative correlation between the percent of retinal ganglion cell loss and the corresponding increased local blood supply after induction of acute high intraocular pressure (r = -0.57 or -0.72) was found. Moreover, a significant negative correlation in the peripheral retina (r = -0.80 or -0.91; p < 0.05) was observed. Conclusion: A disparity exists between changes in local blood supply to the central and middle retina, and the peripheral retina following induction of acute high intraocular pressure in rats. This inequality of changes in local blood supply in rat retina is related to the selective loss of retinal ganglion cells.