Citrus FRIGIDA cooperates with its interaction partnerdehydrin to regulate drought tolerance
Citrus FRIGIDA cooperates with its interaction partnerdehydrin to regulate drought tolerance
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柑橘 FRIGIDA 与其相互作用伙伴脱水素合作调节耐旱性
DOI：
10.1111/tpj.15785
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Jin-Zhi Zhang
中科院分区：
文献类型：
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作者：
Yuan-Yuan Xu;Ren-Fang Zeng;Huan Zhou;Mei-Qi Qiu;Zhi-Meng Gan;Yi-Lin Yang;Si-Fan Hu;Jing-Jing Zhou;Chun-Gen Hu;Jin-Zhi Zhang
干旱是严重影响植物生长和产量的主要环境胁迫，FRI是模式植物开花时间和耐旱性的重要调节因子。然而，关于其在木本植物（包括柑橘）中的功能知之甚少。因此，我们探讨了柑橘FRI同源基因（CiFRI）在干旱下的功能作用。干旱处理诱导了CiFRI的表达，CiFRI的过表达增强了转基因拟南芥和柑橘的耐旱性，而CiFRI的抑制则增加了柑橘的干旱敏感性。此外，干旱条件下的转录组学分析表明，CiFRI过表达改变了参与应激反应、激素生物合成和信号转导的许多基因的表达。机制研究表明，柑橘黄酮可能保护CiFRI免受胁迫诱导的降解，从而增强植物耐旱性。此外，柑橘类抗油菜素唑（BZR）转录因子家族成员（CiBZR 1）在干旱条件下直接与CiFRI启动子结合激活其表达，CiBZR 1也增强了转基因拟南芥和柑橘的耐旱性。这些发现进一步加深了我们对柑橘中CiFRI介导的干旱胁迫反应的分子机制的理解。
Drought is a major environmental stress that severely affects plant growth and crop productivity.FRIGIDA(FRI) is a key regulator of flowering time and drought tolerance in model plants. However, little is known regarding its functions in woody plants, including citrus. Thus, we explored the functional role of the citrusFRIortholog (CiFRI) under drought. Drought treatment inducedCiFRIexpression.CiFRIoverexpression enhanced drought tolerance in transgenic Arabidopsis and citrus, whileCiFRIsuppression increased drought susceptibility in citrus. Moreover, transcriptomic profiling under drought conditions suggested thatCiFRIoverexpression altered the expression of numerous genes involved in the stress response, hormone biosynthesis, and signal transduction. Mechanistic studies revealed that citrus dehydrin likely protects CiFRI from stress‐induced degradation, thereby enhancing plant drought tolerance. In addition, a citrus brassinazole‐resistant (BZR) transcription factor family member (CiBZR1) directly binds to theCiFRIpromoter to activate its expression under drought conditions.CiBZR1also enhanced drought tolerance in transgenic Arabidopsis and citrus. These findings further our understanding of the molecular mechanisms underlying theCiFRI‐mediated drought stress response in citrus.