Neutral monoglycosylceramides in rat brain: Occurrence, molecular expression and developmental variation
Neutral monoglycosylceramides in rat brain: Occurrence, molecular expression and developmental variation
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DOI：
10.1159/000111201
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发表时间：
1997-03-01
影响因子：
2.9
通讯作者：
Hogan, EL
中科院分区：
文献类型：
--
作者：
Dasgupta, S;Everhart, MB;Hogan, EL
研究了中性单糖神经酰胺(MGCS)在胚胎15天(E15)、出生后30天(P30)和成年大鼠脑内的发育性表达。到目前为止，葡萄糖神经酰胺(GlcCer)是唯一在胚胎脑中被鉴定的MGC。半乳糖基神经酰胺(GalCer)在P1时出现，随着年龄的增加而增加，直到P25，此后保持不变。GlcCer和GalCer的发育发生与它们各自的葡萄糖和半乳糖转移酶的活性很好地一致。用气相色谱检测的脑苷脂肪酸和碱的组成在发育过程中也会发生变化。具有比GalCer/GlcCer更高的薄层层析Rf的几种碱不稳定的快速迁移脑苷脂(FMCS)在P10开始表达，浓度随年龄增加而增加(P25-P30)，并保持不变直到成熟。它们都是GalCer的衍生物，通过新开发的中性甲基化程序，我们确认了其中一种FMCS的结构为6-酰基GalCer。在小鼠遗传性髓鞘障碍(Jimpy和颤抖)中，脑FMC浓度和GalCer的降低进一步支持了它们是髓鞘成分的结论。
Developmental expression of neutral monoglycosylceramides (MGCs) has been examined in rat brain from embryonic day 15 (E15) to postnatal day 30 (P30) and adulthood. To this point, glucosylceramide (GlcCer) is the only MGC that has been characterized in embryonic brain. Galactosylceramide (GalCer) appears at P1, increases with age until P25 and remains constant thereafter. The developmental occurrence of GlcCer and GalCer agrees well with their respective glucosyl- and galactosyltransferase activities. Cerebroside fatty-acid and base compositions, examined by gas chromatography, also change during development. Several alkali-labile fast-migrating cerebrosides (FMCs) with a higher thin-layer chromatography RF than GalCer/GlcCer are expressed early at P10, increase in concentration with age (P25-P30) and are unchanged until maturity. They are derivatives of GalCer, By employing a newly developed neutral methylation procedure, we have confirmed the structure of one of the FMCs as 6-acylGalCer. A reduction in brain FMC concentrations along with GalCer in murine genetic dysmyelinating disorders (jimpy and quaking) further supports the conclusion that they are myelin constituents.