Marisediminitalea mangrovi gen. nov., sp. nov., isolated from marine mangrove sediment, and reclassification of Aestuariibacter aggregatus as Marisediminitalea aggregata comb. nov.
Marisediminitalea mangrovi gen. nov., sp. nov., isolated from marine mangrove sediment, and reclassification of Aestuariibacter aggregatus as Marisediminitalea aggregata comb. nov.
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Marisedimitalea mangrovi gen.
DOI：
10.1099/ijsem.0.003773
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发表时间：
2020
影响因子：
2.8
通讯作者：
Xu Hong
中科院分区：
文献类型：
--
作者：
Zhang Danyang;Gui Jiali;Zheng Sisi;Zhu Xiaoying;Wu Shiyin;Tian Yun;Lai Qiliang;Xu Hong
GS-14 T菌株是从广西北仑河口国家级自然保护区的红树林沉积物中分离得到的。细胞为革兰氏染色阴性，严格需氧，杆状，有极鞭毛。最佳生长发生在3- 6%（w/v）NaCl的存在下，在pH 6-8和37 °C的温度下。 主要极性脂质为磷脂酰甘油和磷脂酰乙醇胺。泛醌8（Q-8）是唯一的呼吸醌。主要脂肪酸（>总脂肪酸的10%）为特征3（C16：1ω 7 c和/或C16：1ω6c）和C16：0的总和。 DNA G+C含量为47.6mol%。系统发育分析表明，GS-14 T与AestuariibacteraggregatusWH 169 T、AliglaciecolacorengensisAK 49 T和AlteromonaslipolyticaJW 12 T的同源性最高，分别为96.63%、96.56%和96.22%。 此外，还计算了GS-14 TandA菌株基因组的OrthoANIu值和dDDH值。聚集体WH 169 T分别为79.5%和21.9%。 根据多相分类结果，GS-14 T菌株被认为是一个新属中的一个新种，该新属命名为Marisediminitaleamangrovigen。十一月，sp. nov.的报道。模式菌株为红树林马里斯底虫GS-14 T（=KCTC 72401 T =MCCC 1 K 03622 T）。在系统发育树中，聚集性Aestuariibacter aggregatusWH 169 T与GS-14菌株聚为一簇，并与Aestuariibacter属内的两个种明显分离，因此将其重新分类为Marisediminitalea aggregatacomb属的成员。nov.（模式菌株WH 169 T =CGMCC 1.8995T=LMG 25283 T）。本属的模式种为Marisediminitalea aggregatagen。十一月，梳新
Strain GS-14Twas isolated from a mangrove sediment sample collected at Beilun Estuary National Nature Reserve, Guangxi Province, PR China. Cells were Gram-stain-negative, strictly aerobic and rod-shaped with a polar flagellum. Optimal growth occurred in the presence of 3–6 % (w/v) NaCl, at pH 6–8 and at a temperature of 37 °C. The predominant polar lipids were phosphatidylglycerol and phosphatidylethanolamine. Ubiquinone 8 (Q-8) was the sole respiratory quinone. The major fatty acids (>10 % of the total fatty acids) were summed feature 3 (C16 : 1ω7cand/or C16 : 1ω6c) and C16 : 0. The DNA G+C content was 47.6 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain GS-14Thad the highest sequence similarity toAestuariibacter aggregatusWH169T(96.63 %),Aliiglaciecola coringensisAK49T(96.56 %) andAlteromonas lipolyticaJW12T(96.22 %). In addition, the OrthoANIu value and dDDH values calculated from the genomes of strain GS-14TandA. aggregatusWH169Twere 79.5 and 21.9 %, respectively. Based on the polyphasic taxonomic results, strain GS-14Tis considered to represent a novel species in a new genus, for which the nameMarisediminitalea mangrovigen. nov., sp. nov. is proposed. The type strain ofMarisediminitalea mangroviis GS-14T(=KCTC 72401T=MCCC 1K03622T). BecauseAestuariibacter aggregatusWH169Tclustered with strain GS-14Tin the phylogenetic trees and was clearly separated from the two species within the genusAestuariibacter, it is reclassified as a member of the genusMarisediminitaleaasMarisediminitalea aggregatacomb. nov. (type strain WH169T=CGMCC 1.8995T=LMG 25283T). The type species of the genusMarisediminitaleaisMarisediminitalea aggregatagen. nov., comb. nov.