Paper test card for detection of adulterated milk
Paper test card for detection of adulterated milk
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掺假牛奶检测纸质检测卡
DOI：
10.1039/c7ay01321c
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发表时间：
2017
影响因子：
3.1
通讯作者：
M. Lieberman
中科院分区：
文献类型：
--
作者：
Jamie L. Luther;Valentine Henry de Frahan;M. Lieberman
在发展中国家，牛奶被掺假是为了满足需求和增加利润。通常，牛奶用水稀释，加入糖、尿素和淀粉，以模拟真实牛奶的颜色、稠度和氮含量。由于检测这些掺杂物的实验室测试很难在现场进行，我们开发了一种廉价的纸质分析设备(MilkPAD)来检测牛奶中的这些分析物。进行每项测试所需的所有试剂、缓冲液和酶都以干燥形式储存在试纸中。用户只需将几滴牛奶滴到每个反应区域，等待40分钟，然后用手机拍摄测试卡的照片。该测试卡可以比色检测各种淀粉(0.005%w/v)、尿素(超过70mgdL−1)以及葡萄糖和蔗糖(超过0.1mM)。MilkPAD的灵敏度和特异度超过90%，可检测出通常在掺假牛奶中发现的任何掺杂物。
In developing countries, milk is adulterated to meet demand and increase profit. Often the milk is diluted with water, and sugar, urea, and starch are added to mimic the color, consistency, and nitrogen content of the real thing. Since laboratory tests for detecting these adulterants are difficult to perform in a field setting, we developed an inexpensive, paper analytical device (MilkPAD) to detect these analytes in milk. All of the reagents, buffers, and enzymes needed to perform each test are stored in dry form in the paper. The user just needs to pipette several drops of the milk onto each reaction area, wait 40 minutes, and take a cell phone photo of the test card. The test card can colorimetrically detect a variety of starches (0.005% w/v), urea (in excess of 70 mg dL−1), and the sugars glucose and sucrose (in excess of 0.1 mM). The sensitivity and specificity of the MilkPAD is greater than 90% for detection of any of these adulterants at concentrations that are typically found in adulterated milk.
影响因子：
7.4
作者：
Weaver, Abigail A.;Reiser, Hannah;Barstis, Toni;Benvenuti, Michael;Ghosh, Debarati;Hunckler, Michael;Joy, Brittney;Koenig, Leah;Raddell, Kellie;Lieberman, Marya
通讯作者：
Lieberman, Marya