ABCG2 Gene and ABCG2 Protein Expression in Colorectal Cancer-In Silico and Wet Analysis.
ABCG2 Gene and ABCG2 Protein Expression in Colorectal Cancer-In Silico and Wet Analysis.
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DOI：
10.3390/ijms241310539
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发表时间：
2023-06-23
影响因子：
5.6
通讯作者：
Balcerczak E
中科院分区：
文献类型：
--
作者：
Sałagacka-Kubiak A;Zawada D;Saed L;Kordek R;Jeleń A;Balcerczak E
ABCG 2（ATP结合盒超家族G成员2）是由ABCG 2基因编码的细胞膜泵。ABCG 2可以保护细胞免受引发和/或强化瘤形成的化合物的侵害，并且被认为是负责癌症生长、耐药性和复发的干细胞的标志物。ABCG 2基因或其蛋白的表达已被证明是各种恶性肿瘤的负预后因素。然而，其在结直肠癌中的预后意义仍不清楚。使用公开可用的数据，显示ABCG 2在结肠和直肠腺癌中表达不足，与健康个体的相邻非恶性肺组织和非肿瘤肺组织相比表达较低。这种下调可能是由于ABCG 2基因某些位点的甲基化水平。这与结肠腺癌患者的微卫星不稳定性、体重和年龄有关，与直肠腺癌患者的肿瘤定位、群体类型和年龄有关。未发现ABCG 2表达水平与结直肠癌患者的生存率之间存在相关性。在结直肠癌样本的湿分析中，通过RT-PCR分析的ABCG 2基因表达和通过免疫组织化学评估的ABCG 2蛋白水平均与任何临床病理因素或总生存期无关。STRING构建的以ABCG 2为中心的蛋白质-蛋白质相互作用网络显示，蛋白质被发现参与白三烯、有机阴离子和外源性物质的转运、内胚层细胞命运的指定以及组蛋白甲基化和泛素化。因此，ABCG 2低表达可能是某些信号通路或蛋白质相互作用的活性指标，对结直肠癌的发生至关重要。
ABCG2 (ATP-binding cassette superfamily G member 2) is a cell membrane pump encoded by the ABCG2 gene. ABCG2 can protect cells against compounds initiating and/or intensifying neoplasia and is considered a marker of stem cells responsible for cancer growth, drug resistance and recurrence. Expression of the ABCG2 gene or its protein has been shown to be a negative prognostic factor in various malignancies. However, its prognostic significance in colorectal cancer remains unclear. Using publicly available data, ABCG2 was shown to be underexpressed in colon and rectum adenocarcinomas, with lower expression compared to both the adjacent nonmalignant lung tissues and non-tumour lung tissues of healthy individuals. This downregulation could result from the methylation level of some sites of the ABCG2 gene. This was connected with microsatellite instability, weight and age among patients with colon adenocarcinoma, and with tumour localization, population type and age of patients for rectum adenocarcinoma. No association was found between ABCG2 expression level and survival of colorectal cancer patients. In wet analysis of colorectal cancer samples, neither ABCG2 gene expression, analysed by RT-PCR, nor ABCG2 protein level, assessed by immunohistochemistry, was associated with any clinicopathological factors or overall survival. An ABCG2-centered protein–protein interaction network build by STRING showed proteins were found to be involved in leukotriene, organic anion and xenobiotic transport, endodermal cell fate specification, and histone methylation and ubiquitination. Hence, ABCG2 underexpression could be an indicator of the activity of certain signalling pathways or protein interactors essential for colorectal carcinogenesis.
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ABCG2维持的卟啉稳态调节胚胎干细胞的自我更新。
影响因子：
3.7
作者：
Susanto J;Lin YH;Chen YN;Shen CR;Yan YT;Tsai ST;Chen CH;Shen CN
通讯作者：
Shen CN
影响因子：
7.3
作者：
Diestra, JE;Scheffer, GL;Izquierdo, MA
通讯作者：
Izquierdo, MA
影响因子：
5.8
作者：
Gheytanchi E;Naseri M;Karimi-Busheri F;Atyabi F;Mirsharif ES;Bozorgmehr M;Ghods R;Madjd Z
通讯作者：
Madjd Z
影响因子：
3.8
作者：
Andersen V;Ostergaard M;Christensen J;Overvad K;Tjønneland A;Vogel U
通讯作者：
Vogel U
DOI：
10.1016/j.bbrc.2006.02.172
发表时间：
2006-05-05
影响因子：
3.1
作者：
Gupta, N;Martin, PM;Ganapathy, V
通讯作者：
Ganapathy, V