Models of oral and vaginal candidiasis based on in vitro reconstituted human epithelia for the study of host-pathogen interactions.
Models of oral and vaginal candidiasis based on in vitro reconstituted human epithelia for the study of host-pathogen interactions.
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基于体外重建人类上皮的口腔和阴道念珠菌病模型，用于研究宿主与病原体的相互作用。
DOI：
10.1007/978-1-59745-204-5_23
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Weindl,Gunther
中科院分区：
文献类型：
--
作者：
Schaller,Martin;Weindl,Gunther
This protocol describes the setup, maintenance, and characteristics of models of oral and vaginal candidiasis based on well-established three-dimensional organotypic tissues of human oral and vaginal mucosa. Infection experiments are highly reproducible and can be used for the direct analysis of pathogen/epithelial cell interactions. Using the models, the several stages of infection by wild-typeCandida albicansstrains, the consequence of gene disruption of putative virulence factors in mutant cells, and the evaluation of the host immune response can be evaluated by histologic, biochemical, and molecular methods. As such, the models provide clear answers regarding protein and gene expression that are not complicated by nonepithelial factors. To study the impact of several host components, the mucosal infection models can be supplemented with immune cells, saliva, and probiotic bacteria, which might be relevant for host defense. It requires at least 3 days to be established and can be maintained thereafter for 2 to 4 days.
