TRANSPLACENTAL TRANSMISSION OF POLYOMA-VIRUS IN MICE
TRANSPLACENTAL TRANSMISSION OF POLYOMA-VIRUS IN MICE
复制标题
DOI：
10.1128/iai.18.1.196-202.1977
复制
发表时间：
1977-01-01
影响因子：
3.1
通讯作者：
MIMS, CA
中科院分区：
文献类型：
--
作者：
MCCANCE, DJ;MIMS, CA
当妊娠小鼠在妊娠第1天接种多发性肿瘤时，其中一些表现为完全吸收或产仔数减少，程度取决于病毒剂量。在11个感染母亲的小鼠胚胎成纤维细胞(MEF)培养物中，有4个检测到病毒。母体感染后第5天或第10天，所有检测的胎鼠血清中病毒滴度均达10750%组织培养感染量(TCID50)/克胎儿，第6天下降，第6天出现血凝抑制抗体，第14天最高。第7天检测到免疫球蛋白G类抗体，滴度在第14天迅速上升。妊娠晚期(第15天)母体感染后，1/3的新生小鼠肾脏样本中有105TCID50多瘤病毒。
When pregnant mice were inoculated on day 1 of gestation with polyoma, some of them exhibited total resorption or reduced litter size, the extent depending on virus dose. Virus was detected in 4 of 11 mouse embryo fibroblast (MEF) cultures made from infected mothers. After maternal infection on day 5 or 10 of gestation, virus titers of up to 107 50% tissue culture infectious doses (TCID50)/g of fetus were found in all pools of fetuses tested 5 days later, with the titers falling by day 6. Hemagglutination-inhibiting antibodies against polyoma appeared in maternal serum by day 6 and rose to a maximum by day 14. Immunoglobulin G class antibodies were detected by day 7, with titers rising rapidly to a maximum at day 14. After maternal infection later in gestation (day 15), 1 of 3 litters of newborn mice had 105 TCID50 polyoma virus/g in pooled kidney samples.