Cr(VI) Induces Lipid Peroxidation, Protein Oxidation and Alters the Activities of Antioxidant Enzymes in Human Erythrocytes
Cr(VI) Induces Lipid Peroxidation, Protein Oxidation and Alters the Activities of Antioxidant Enzymes in Human Erythrocytes
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DOI：
10.1007/s12011-011-9119-5
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发表时间：
2011-12-01
影响因子：
3.9
通讯作者：
Mahmood, Riaz
中科院分区：
文献类型：
--
作者：
Ahmad, Mir Kaisar;Syma, Syed;Mahmood, Riaz
在体外条件下，研究了六价铬化合物重铬酸钾（K2Cr2O7）对人红细胞的影响。用不同浓度的K2Cr2O7孵育红细胞可导致明显的溶血，并呈浓度依赖性。K2Cr2O7处理还引起蛋白氧化、脂质过氧化显著增加，总巯基含量显著降低，提示其引起人红细胞氧化应激。然而，由于k2cr2o7处理的红细胞溶血物中的一氧化氮水平低于对照组，因此没有伴随的亚硝化应激。K2Cr2O7使红细胞过氧化氢酶、谷胱甘肽过氧化物酶、硫氧还蛋白还原酶、葡萄糖-6-磷酸脱氢酶和谷胱甘肽还原酶活性降低，而铜锌超氧化物歧化酶和谷胱甘肽s -转移酶活性升高。这些结果表明，K2Cr2O7可诱导氧化应激，改变人红细胞的抗氧化防御机制。
The effect of potassium dichromate (K2Cr2O7), a hexavalent chromium compound, on human erythrocytes was studied under in vitro conditions. Incubation of erythrocytes with different concentrations of K2Cr2O7 resulted in marked hemolysis in a concentration-dependent manner. K2Cr2O7 treatment also caused significant increase in protein oxidation, lipid peroxidation and decrease in total sulfhydryl content, indicating that it causes oxidative stress in human erythrocytes. However, there was no concomitant nitrosative stress as the nitric oxide levels in hemolysates from K2Cr2O7-treated erythrocytes were lower than in control. Exposure of erythrocytes to K2Cr2O7 decreased the activities of catalase, glutathione peroxidase, thioredoxin reductase, glucose-6-phosphate dehydrogenase, and glutathione reductase, whereas the activities of Cu-Zn superoxide dismutase and glutathione S-transferase were increased. These results show that K2Cr2O7 induces oxidative stress and alters the antioxidant defense mechanism of human erythrocytes.