Phage Displayed Peptides to Avian H5N1 Virus Distinguished the Virus from Other Viruses
Phage Displayed Peptides to Avian H5N1 Virus Distinguished the Virus from Other Viruses
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噬菌体展示禽 H5N1 病毒肽，将该病毒与其他病毒区分开来
DOI：
10.1371/journal.pone.0023058
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发表时间：
2011-08-22
期刊：
影响因子：
3.7
通讯作者：
Ren, Xiaofeng
中科院分区：
文献类型：
--
作者：
Wu, Dan;Li, Guangxing;Ren, Xiaofeng
本研究的目的是利用噬菌体展示技术筛选高致病性禽流感病毒（H5 N1亚型）的配体，并建立一种新的诊断方法。H5 N1病毒在使用12-mer噬菌体展示随机肽库的生物淘选过程中用作固定化靶标。经过5轮淘选，分离到3个表达H5 N1病毒特异性结合活性的肽HAWDPIPARDPF、AAWHLIVALAPN或ATSHLHVRLPSK。通过DNA测序鉴定了H5 N1病毒的推定结合基序。在最小病毒量方面，噬菌体ELISA优于抗血清ELISA和人工半定量终点RT-PCR检测H5 N1病毒。更重要的是，所选择的带有H5 N1病毒特异性肽的抗体能够在酶联免疫吸附试验中将该病毒与其他禽类病毒区分开。
The purpose of the current study was to identify potential ligands and develop a novel diagnostic test to highly pathogenic avian influenza A virus (HPAI), subtype H5N1 viruses using phage display technology. The H5N1 viruses were used as an immobilized target in a biopanning process using a 12-mer phage display random peptide library. After five rounds of panning, three phages expressing peptides HAWDPIPARDPF, AAWHLIVALAPN or ATSHLHVRLPSK had a specific binding activity to H5N1 viruses were isolated. Putative binding motifs to H5N1 viruses were identified by DNA sequencing. In terms of the minimum quantity of viruses, the phage-based ELISA was better than antiserum-based ELISA and a manual, semi-quantitative endpoint RT-PCR for detecting H5N1 viruses. More importantly, the selected phages bearing the specific peptides to H5N1 viruses were capable of differentiating this virus from other avian viruses in enzyme-linked immunosorbent assays.