Deposition of histones onto replicating chromosomes.
Deposition of histones onto replicating chromosomes.
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组蛋白沉积到复制染色体上。
DOI：
10.1073/pnas.72.11.4440
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发表时间：
1975
影响因子：
11.1
通讯作者：
R. Chalkley
中科院分区：
文献类型：
--
作者：
V. Jackson;D. Granner;R. Chalkley
新合成的和预先存在的组蛋白的分布模式已在染色体复制过程中进行了研究。新合成的组蛋白用[3H]赖氨酸标记，新合成的DNA用碘脱氧尿苷密度标记。将组蛋白共价连接到DNA上，并在CsCl密度梯度上分析放射性标记的组蛋白。我们已经定义了在分离过程中不会引起组蛋白随机化的条件，并且还开发了一种在不可避免地存在非组蛋白的情况下定义组蛋白在染色质中密度梯度分布的方法。可以设想三种可能的模式分布的组蛋白到复制染色体上，我们描述的实验设计，以明确区分这些可能性，并得出结论，组蛋白随机沉积到染色体上。
The mode of distribution of newly synthesized and pre-existing histones has been studied during the process of chromosome replication. Newly synthesized histone was labeled with [3H]lysine and newly synthesized DNA was density labeled with iododeoxyuridine. The histone was covalently linked to DNA, and radiolabeled histone was analyzed on CsCl density gradients. We have defined conditions that do not give rise to histone randomization during isolation, and also developed a method of defining the distribution of histones in chromatin on a density gradient in the unavoidable presence of nonhistone protein. Three possible modes of distribution of histone onto the replicating chromosome can be conceived; we describe experiments designed to distinguish unequivocally among these possibilities and conclude that histones are deposited randomly onto the chromosome.