Identity in molecular structure between "differentiation enhancing factor" of murine erythroleukemia cells and the 30 kD heparin-binding protein of developing rat brain.
Identity in molecular structure between "differentiation enhancing factor" of murine erythroleukemia cells and the 30 kD heparin-binding protein of developing rat brain.
复制标题
小鼠红白血病细胞的“分化增强因子”与发育中的大鼠大脑的 30 kD 肝素结合蛋白之间的分子结构同一性。
DOI：
10.1006/bbrc.1995.1630
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发表时间：
1995
影响因子：
3.1
通讯作者：
S. Pontremoli
中科院分区：
文献类型：
--
作者：
E. Melloni;B. Sparatore;M. Patrone;A. Pessino;M. Passalacqua;S. Pontremoli
先前从鼠红白血病（MEL）细胞中分离出的一种29 kD的蛋白质，显示出增强这些细胞的分化速率，现在已经证明其具有与来自发育中的大鼠脑的30 kD肝素结合蛋白质所报道的相同的氨基酸序列，该蛋白质以其高度偶极结构命名为异丙肾上腺素。这两种蛋白质之间的同一性已经建立在强肝素结合亲和力和N-末端区域以及几种胰蛋白酶肽的氨基酸序列完全同源的基础上。此外，编码该蛋白质的cDNA已经通过逆转录酶-聚合酶链反应从MEL细胞mRNA中分离出来，并且发现其序列与Escherin的序列相对应。MEL细胞分化增强因子（以前缩写为DEF）再次被证实可缩短六亚甲基双乙酰胺诱导的细胞定型出现之前的潜伏期，并刺激α-蛋白激酶C的催化活性。因此，在这里，我们证明了在MEL细胞中表达的蛋白质，其序列与先前报道的Escherin相同，在促进细胞分化中起着至关重要的作用，从而表明Escherin的新的相关功能。
A 29 kD protein previously isolated from murine erythroleukemia (MEL) cells and shown to enhance the rate of differentiation of these cells has now been demonstrated to possess an amino acid sequence identical to that reported for the 30 kD heparin-binding protein from developing rat brain, named amphoterin after its highly dipolar structure. The identity between the two proteins has been established on the basis of a strong heparin binding affinity and a complete homology in the amino acid sequences of N-terminal region as well as of several tryptic peptides. Furthermore, the cDNA encoding this protein has been isolated from MEL cell mRNA, by means of reverse transcriptase-polymerase chain reaction, and its sequence was found to correspond to that of amphoterin. The MEL cell differentiation enhancing factor, previously abbreviated as DEF, is again confirmed to reduce the latent period preceding the appearance of hexamethylenebisacetamide induced cell commitment and to stimulate the catalytic activity of alpha-protein kinase C. Thus, here we demonstrate that a protein expressed in MEL cells, whose sequence is identical to that previously reported for amphoterin, plays an essential role in promoting cell differentiation, thereby indicating a new relevant function of amphoterin.