Dysfunctionality of a tobacco mosaic virus movement protein mutant mimicking threonine 104 phosphorylation
Dysfunctionality of a tobacco mosaic virus movement protein mutant mimicking threonine 104 phosphorylation
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DOI：
10.1099/vir.0.18972-0
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发表时间：
2003-03-01
影响因子：
3.8
通讯作者：
Atabekov, JG
中科院分区：
文献类型：
--
作者：
Karger, EM;Frolova, OY;Atabekov, JG
烟草花叶病毒(TMV)的复制在侵染早期与内质网(ER)相关膜相连。本研究报告了烟草花叶病毒运动蛋白(MP)特异性蛋白激酶(PKs)与烟草ER相关，能够磷酸化TMV MP中的Thr(104)。凝胶PK分析显示MP特异性PKs的表观分子质量分别为45-50 kDa和38 kDa。在野生型TMV U1基因组MP基因中引入两种类型的突变，以中性Ala和带负电荷的Asp取代Thr(104)。Thr(104)突变为Ala不影响突变病毒在烟草中诱导的坏死灶的大小。植物。相反，模拟Thr(104)磷酸化的Thr突变为Asp强烈地抑制了细胞间的运动。讨论了Thr(104)磷酸化在TMV MP功能中的可能作用。
Replication of tobacco mosaic virus (TMV) is connected with endoplasmic reticulum (ER)associated membranes at early stages of infection. This study reports that TMV movement protein (MP)-specific protein kinases (PKs) associated with the ER of tobacco were capable of phosphorylating Thr(104) in TMV MP. The MP-specific PKs with apparent molecular masses of about 45-50 kDa and 38 kDa were revealed by gel PK assays. Two types of mutations were introduced in TMV MP gene of wild-type TMV U1 genome to substitute Thr(104) by neutral Ala or by negatively charged Asp. Mutation of Thr(104) to Ala did not affect the size of necrotic lesions induced by the mutant virus in Nicotiana tabacum, Xanthi nc. plants. Conversely, mutation of Thr to Asp mimicking Thr(104) phosphorylation strongly inhibited cell-to-cell movement. The possible role of Thr(104) phosphorylation in TMV MP function is discussed.