Production of 3-hydroxypropionic acid from glucose and xylose by metabolically engineered Saccharomyces cerevisiae.
Production of 3-hydroxypropionic acid from glucose and xylose by metabolically engineered Saccharomyces cerevisiae.
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DOI：
10.1016/j.meteno.2015.10.001
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发表时间：
2015-12
影响因子：
5.2
通讯作者：
Borodina I
中科院分区：
文献类型：
--
作者：
Kildegaard KR;Wang Z;Chen Y;Nielsen J;Borodina I
生物质是地球上最丰富的碳源，未来可能取代化石原料，成为生产燃料和化学品的主要原料。然而，这将需要开发能够将木质纤维素生物质中存在的C6和C5糖转化为感兴趣的产品的细胞工厂。我们对酿酒酵母进行了改造，使其能够从葡萄糖和木糖中生产3-羟基丙酸（3HP），这是丙烯酸酯的潜在组成部分。我们通过丙二酰辅酶a或β-丙氨酸中间体将3HP生物合成途径引入木糖消耗酵母中。采用控制投料分批培养，120小时获得7.37±0.17 g 3HP L−1木糖，总产量为29±1% Cmol 3HP Cmol−1木糖。这项研究首次证明了利用酿酒酵母从生物质糖木糖中生产3HP的潜力。3HP是丙烯酸塑料和可生物降解聚酯的潜在基石。我们开发了酿酒葡萄球菌菌株，用于从葡萄糖和木糖中生产3HP。通过β-丙氨酸的3HP途径在木糖上产生最高的3HP滴度。产率为29±1% Cmol 3HP Cmol−1，得到7.4±0.2 g 3HP L−1木糖。利用生物质木糖生产3HP的潜力论证。
Biomass, the most abundant carbon source on the planet, may in the future become the primary feedstock for production of fuels and chemicals, replacing fossil feedstocks. This will, however, require development of cell factories that can convert both C6 and C5 sugars present in lignocellulosic biomass into the products of interest. We engineered Saccharomyces cerevisiae for production of 3-hydroxypropionic acid (3HP), a potential building block for acrylates, from glucose and xylose. We introduced the 3HP biosynthetic pathways via malonyl-CoA or β-alanine intermediates into a xylose-consuming yeast. Using controlled fed-batch cultivation, we obtained 7.37±0.17 g 3HP L−1 in 120 hours with an overall yield of 29±1% Cmol 3HP Cmol−1 xylose. This study is the first demonstration of the potential of using S. cerevisiae for production of 3HP from the biomass sugar xylose. 3HP is a potential building block for acrylic plastics and biodegradable polyesters. We developed S. cerevisiae strains for production of 3HP from glucose and xylose. 3HP pathway via β-alanine resulted in the highest 3HP titers on xylose. 7.4±0.2 g 3HP L−1 was obtained with a yield of 29±1% Cmol 3HP Cmol−1 xylose. Demonstration of the potential of using the biomass sugar xylose to produce 3HP.