Enhancer-promoter specificity mediated by DPE or TATA core promoter motifs
Enhancer-promoter specificity mediated by DPE or TATA core promoter motifs
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DOI：
10.1101/gad.924301
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发表时间：
2001-10-01
影响因子：
10.5
通讯作者：
Kadonaga, JT
中科院分区：
文献类型：
--
作者：
Butler, JEF;Kadonaga, JT
为了研究增强子-启动子特异性的基础，我们比较了增强子在体内从含有下游启动子元件（DPE）或TATA盒基序的核心启动子激活转录的能力。为了消除位置效应，我们生成并分析了成对的姐妹果蝇品系，这些品系含有DPE或TATA依赖性报告基因，相对于每个增强子位于完全相同的基因组位置。这些研究揭示了对含有DPE或TATA盒元件的启动子具有特异性的转录增强子。因此，核心启动子不仅介导转录的起始，而且还作为调控元件起作用。
To investigate the basis for enhancer-promoter specificity, we compared the ability of enhancers to activate transcription in vivo from core promoters containing either downstream promoter element (DPE) or TATA box motifs. To eliminate position effects, we generated and analyzed pairs of sister Drosophila lines that contain a DPE- or TATA-dependent reporter gene at precisely the same genomic position relative to each enhancer. These studies revealed transcriptional enhancers that are specific for promoters that contain either DPE or TATA box elements. Thus, the core promoter not only mediates the initiation of transcription, but also functions as a regulatory element.