Osteoblast-like cell mineralization induced by multiphasic calcium phosphate ceramic
Osteoblast-like cell mineralization induced by multiphasic calcium phosphate ceramic
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DOI：
10.1016/j.msec.2005.08.028
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发表时间：
2006-09-01
期刊：
影响因子：
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通讯作者：
Kleebe, Hans-Joachim
中科院分区：
文献类型：
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作者：
Ayers, Reed;Nielsen-Preiss, Sheila;Kleebe, Hans-Joachim
这里介绍的工作检查了多相 CaP 材料对 SaOS-2 成骨细胞样细胞活性的影响。采用自蔓延高温燃烧合成法（SHS）合成多相磷酸钙（HCaP）。所有样品均使用 XRD、SEM 和 FTIR 进行表征。 HCaP 与 SaOS-2 细胞的细胞培养程序遵循 ASTM 813-01。通过环境扫描电镜 (ESEM) 和 EDS 检查样品，以表征培养物的矿物质含量。镀金样品用于高分辨率成像。在暴露于 HCaP 的未条件培养物中注意到生物矿化，而对照（仅在培养基中的细胞和仅在培养基中的 HCaP）未显示矿化。在表达含有磷酸钙的囊泡的细胞附近鉴定出磷酸钙板状结构。这些结构与其他已发表的作品中看到的有机磷酸盐晶体相似。在 SaOS-2 细胞的较大膜囊泡中发现板状晶体，表明细胞介导的生物矿化的发生。 (c) 2005 Elsevier B.V. 保留所有权利。
The work presented here examines the effect of multiphasic CaP materials on the activity of SaOS-2 osteoblast like cells. Heterogeneous calcium phosphate (HCaP) was synthesized using self-propagating high temperature combustion synthesis (SHS). All samples were characterized using XRD, SEM, and FTIR. Cell culture procedures of HCaP with SaOS-2 cells followed ASTM 813-01. Samples were examined by Environmental SEM (ESEM) and EDS to characterize the mineral content of the cultures. Gold-coated samples were used for high-resolution imaging. Biomineralization was noted in unconditioned cultures exposed to HCaP while the controls (cells only in media and HCaP only in media) showed no mineralization. Calcium phosphate plate-like structures were identified adjacent to cells expressing calcium phosphate containing vesicles. These structures are similar to the organophosphate crystals seen in other published works. Plate-like crystals were noted in the larger membrane vesicles of SaOS-2 cells indicating the occurrence of cellularly mediated biomineralization. (c) 2005 Elsevier B.V. All rights reserved.