Cyclical Cell Stretching of Skin-Derived Fibroblasts Downregulates Connective Tissue Growth Factor (CTGF) Production
Cyclical Cell Stretching of Skin-Derived Fibroblasts Downregulates Connective Tissue Growth Factor (CTGF) Production
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DOI：
10.3109/03008200902836081
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发表时间：
2009-01-01
影响因子：
2.9
通讯作者：
Ichinose, Masaharu
中科院分区：
文献类型：
--
作者：
Kanazawa, Yuichiro;Nomura, Jun;Ichinose, Masaharu
皮肤伤口的延迟愈合可由伤口不稳定引起，而适当的按摩或运动可防止硬化和瘢痕挛缩。然而，创伤愈合与机械应力相关的机制尚未完全阐明。本研究旨在确定成纤维细胞的机械拉伸是否会减少其细胞外基质的产生。我们将皮肤成纤维细胞转移到胶原包被的弹性硅胶腔中，在拉伸装置上培养它们，并使用RT-PCR检测机械拉伸对与细胞外基质产生和生长因子分泌相关的17个基因表达水平的影响。我们发现，结缔组织生长因子（CTGF）下调后24小时的细胞拉伸。具体而言，CTGF mRNA和蛋白水平分别为对照水平的50%和48%。这些发现表明成纤维细胞的周期性拉伸通过减少CTGF产生而有助于抗纤维化过程。
Delayed healing of skin wounds can be caused by wound instability, whereas appropriate massage or exercise prevents sclerosis and scar contracture. However, the mechanism by which wound healing is related to mechanical stress has not been fully elucidated. The present study aimed to identify whether mechanical stretching of fibroblasts reduces their production of extracellular matrix. We transferred skin fibroblasts into collagen-coated elastic silicone chambers, cultured them on a stretching apparatus, and used RT-PCR to examine the effects of mechanical stretching on the expression levels of 17 genes related to extracellular matrix production and growth factor secretion. We found that connective tissue growth factor (CTGF) was downregulated after 24 hr of cell stretching. Specifically, the CTGF mRNA and protein levels were 50% and 48% of the control levels, respectively. These findings suggest that cyclic stretching of fibroblasts contributes to anti-fibrotic processes by reducing CTGF production.