Pomegranate juice and punicalagin attenuate oxidative stress and apoptosis in human placenta and in human placental trophoblasts
Pomegranate juice and punicalagin attenuate oxidative stress and apoptosis in human placenta and in human placental trophoblasts
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DOI：
10.1152/ajpendo.00003.2012
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发表时间：
2012-05-01
影响因子：
5.1
通讯作者：
Nelson, D. Michael
中科院分区：
文献类型：
--
作者：
Chen, Baosheng;Tuuli, Methodius G.;Nelson, D. Michael
陈斌，陈丽丽，陈建军，陈建军，陈建军，陈建军。石榴汁和石榴苷对人胎盘滋养细胞氧化应激和细胞凋亡的影响。[J] .中华内分泌杂志，2002,26(1):1 - 4。首次发表于2012年2月28日；doi: 10.1152 / ajpendo.00003.2012。人类胎盘是妊娠结局的关键，在许多复杂妊娠中存在的氧化应激升高会导致胎盘功能障碍和次优妊娠结局。我们对富含多酚类抗氧化剂的石榴汁在体内和体外限制胎盘滋养细胞损伤的假设进行了验证。单胎妊娠的孕妇在妊娠35周至38周时被随机分配到8盎司/天的石榴汁或苹果汁（安慰剂），直到分娩。收集12例患者的胎盘组织（石榴组4例，对照组8例）进行氧化应激分析。体内初步结果扩展到体外氧化应激和细胞死亡试验。在规定的氧张力和化学刺激下，将胎盘外植体和培养的原代人滋养细胞暴露于石榴汁或葡萄糖（对照）中。我们发现产前饮用石榴汁后分娩的孕妇足月胎盘的氧化应激降低，而苹果汁作为对照。此外，石榴汁可以减少体外氧化应激、细胞凋亡以及暴露于缺氧、模拟缺氧的氯化钴和激酶抑制剂staurosporine的足月绒毛外植体和原代滋养细胞培养物的整体细胞死亡。石榴汁中两种主要的多酚类物质——石榴苷，而不是鞣花酸，可以降低培养的合胞滋养细胞的氧化应激和刺激诱导的凋亡。我们得出结论，石榴汁在体内和体外降低胎盘氧化应激，同时限制刺激诱导的培养人滋养细胞死亡。多酚槟榔苷模拟了这种保护作用。我们推测，产前摄入石榴可能限制胎盘损伤，从而可能对暴露的胎儿提供保护。
Chen B, Tuuli MG, Longtine MS, Shin JS, Lawrence R, Inder T, Nelson DM. Pomegranate juice and punicalagin attenuate oxidative stress and apoptosis in human placenta and in human placental trophoblasts. Am J Physiol Endocrinol Metab 302: E1142-E1152, 2012. First published February 28, 2012; doi:10.1152/ajpendo.00003.2012.-The human placenta is key to pregnancy outcome, and the elevated oxidative stress present in many complicated pregnancies contributes to placental dysfunction and suboptimal pregnancy outcomes. We tested the hypothesis that pomegranate juice, which is rich in polyphenolic antioxidants, limits placental trophoblast injury in vivo and in vitro. Pregnant women with singleton pregnancies were randomized at 35 similar to 38 wk gestation to 8 oz/day of pomegranate juice or apple juice (placebo) until the time of delivery. Placental tissues from 12 patients (4 in the pomegranate group and 8 in the control group) were collected for analysis of oxidative stress. The preliminary in vivo results were extended to oxidative stress and cell death assays in vitro. Placental explants and cultured primary human trophoblasts were exposed to pomegranate juice or glucose (control) under defined oxygen tensions and chemical stimuli. We found decreased oxidative stress in term human placentas from women who labored after prenatal ingestion of pomegranate juice compared with apple juice as control. Moreover, pomegranate juice reduced in vitro oxidative stress, apoptosis, and global cell death in term villous explants and primary trophoblast cultures exposed to hypoxia, the hypoxia mimetic cobalt chloride, and the kinase inhibitor staurosporine. Punicalagin, but not ellagic acid, both prominent polyphenols in pomegranate juice, reduced oxidative stress and stimulus-induced apoptosis in cultured syncytiotrophoblasts. We conclude that pomegranate juice reduces placental oxidative stress in vivo and in vitro while limiting stimulus-induced death of human trophoblasts in culture. The polyphenol punicalagin mimics this protective effect. We speculate that antenatal intake of pomegranate may limit placental injury and thereby may confer protection to the exposed fetus.