Electrochemically induced surface-enhanced infrared difference absorption (SEIDA) spectroscopy of a protein monolayer
Electrochemically induced surface-enhanced infrared difference absorption (SEIDA) spectroscopy of a protein monolayer
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DOI：
10.1021/ja0346532
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发表时间：
2003-04-30
影响因子：
15
通讯作者：
Heberle, J
中科院分区：
文献类型：
--
作者：
Ataka, K;Heberle, J
功能性红外差光谱的蛋白质单层记录利用改性金表面所施加的表面增强。贵金属被用作工作电极，以诱导细胞色素的单层中的氧化还原相关的结构变化。所观察到的相对带强度的差异相比，细胞色素蛋白质溶液的差异光谱是由于吸附的蛋白质的取向效应。
Functional infrared difference spectra of a protein monolayer are recorded by exploiting the surface enhancement exerted by a modified gold surface. The noble metal is used as the working electrode to induce redox-linked structural changes in the single layer of cytochromec. The observed differences in the relative band intensities as compared to the difference spectrum of cytochromecin solution are attributed to orientational effects of the adsorbed protein.