Application of a novel nylon needle filter-based solid-phase extraction device to determination of 1-hydroxypyrene in urine.
Application of a novel nylon needle filter-based solid-phase extraction device to determination of 1-hydroxypyrene in urine.
复制标题
新型尼龙针过滤器固相萃取装置测定尿液中1-羟基芘的应用
DOI：
10.1002/jssc.202100830
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发表时间：
2022-01
影响因子：
3.1
通讯作者：
Qin Zhao
中科院分区：
文献类型：
--
作者：
Shi-Wen Yue;Yi-Lian Zhou;Xi-Tian Peng;Qin Zhao
本工作将市售尼龙针状过滤器与注射器连接，构建了一种简单、小型化的固相萃取装置，用于通过疏水作用和氢键作用从尿样中提取1-羟基芘。针刺过滤器中的尼龙膜作为固相萃取吸附剂，同时过滤尿样中的颗粒。为获得较高的萃取效率，考察了影响萃取率的关键参数。在最佳条件下，整个前处理过程在5min内完成。采用高效液相色谱-紫外联用技术，建立了一种快速、低成本、简便的尼龙针刺滤膜分析复杂尿液基质中1-羟基芘的方法。方法在0.21000μg/L范围内线性关系良好(R=0.9999)。方法的检出限为0.06μg/L，添加5、20、100μg/L三种浓度的尿样的回收率在105.8%~113.1%之间，日内和日间RSD分别小于6.7%和8.9%。最后，将该方法成功地应用于大学生、吸烟者、加油站工作人员和芯片厂工人尿样中1-羟基芘的检测。实际尿样中的检测浓度范围为0.46~5.2 6μg/L。综上所述，这种简单、经济的尼龙针滤固相萃取装置在水样中疏水性分析物质的前处理中显示出良好的应用潜力。这篇文章受版权保护。版权所有。
In this work, a simple and miniaturized solid-phase extraction device was constructed by connecting a commercial nylon needle filter to a syringe, which was applied for extracting 1-hydroxypyrene from urine sample via hydrophobic and hydrogen bond interactions. The nylon membrane in the needle filter acted as the solid-phase extraction adsorbent, meanwhile, it filtered the particles in the urine sample. To obtain high extraction efficiency, key parameters influencing extraction recovery were investigated. The entire pretreatment process was accomplished within 5 min under the optimal conditions. By coupling HPLC-UV, a rapid, low-cost, and convenient nylon needle filter-based method was established for the analysis of 1-hydroxypyrene in complex urine matrix. Within the linearity range of 0.2-1000 μg/L, the method exhibited a satisfactory correlation coefficient (R = 0.9999). The limit of detection was 0.06 μg/L, and the recoveries from urine sample spiked with three concentrations (5, 20, and 100 μg/L) ranged from 105.8% to 113.1% with the relative standard deviations less than 6.7% (intra-day, n = 6) and 8.9% (inter-day, n = 4). Finally, the proposed method was successfully applied for detecting 1-hydroxypyrene in urine samples from college students, smokers, gas station workers, and chip factory workers. The detected concentration in actual urine samples ranged from 0.46 to 5.26 μg/L. Taken together, this simple and cost-effective nylon needle filter-based solid-phase extraction device showed an excellent application potential for pretreating hydrophobic analytes from aqueous samples. This article is protected by copyright. All rights reserved.