THE RELATIONSHIP BETWEEN MESSENGER-RNA STABILITY AND LENGTH IN SACCHAROMYCES-CEREVISIAE
THE RELATIONSHIP BETWEEN MESSENGER-RNA STABILITY AND LENGTH IN SACCHAROMYCES-CEREVISIAE
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DOI：
10.1093/nar/14.21.8347
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发表时间：
1986-11-11
影响因子：
14.9
通讯作者：
BROWN, AJP
中科院分区：
文献类型：
--
作者：
SANTIAGO, TC;PURVIS, IJ;BROWN, AJP
A rapid and convenient procedure has been developed for the measurement of mRNA half-life inS.cerevisiaeusing the transcriptional inhibitor, 1,10-phenanthroline. A range of half-lives from 6.6 ± 0.67 minutes to over 100 minutes, relative to the stability of the 18S rRNA control, has been obtained for fifteen mRNAs. They include the pyruvate kinase and actin mRNAs, as well as 13 randomly picked mRNAs of unknown function. The mRNAs clearly fall into two populations when their lengths and half-lives are analysed; one population is considerably more stable than the other when mRNAs of similar length are compared. Also, within each population, there is an inverse relationship between mRNA length and half-life. These results suggest that mRNA length and at least one additional factor strongly influence mRNA stability in yeast.
