Modulated Growth of Nanoparticles. Application for Sensing Nerve Gases
Modulated Growth of Nanoparticles. Application for Sensing Nerve Gases
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DOI：
10.1021/ac801949x
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发表时间：
2009-01-01
影响因子：
7.4
通讯作者：
Pavlov, Valeri
中科院分区：
文献类型：
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作者：
Virel, Ana;Saa, Laura;Pavlov, Valeri
由乙酰胆碱酯酶介导的乙酰硫代胆碱的水解产生带有硫醇的化合物硫代胆碱。在抗坏血酸存在的情况下，痕量浓度的硫代胆碱可调节金纳米颗粒在金纳米颗粒上的生长。通过 1,5-双(4-烯丙基二甲基铵苯基)戊-3-酮二溴化物 (BW284c51) 或二乙基对硝基苯磷酸酯 (对氧磷) 抑制该酶，会产生较低的硫代胆碱产量，从而促进金银纳米粒子的催化生长。在这里，我们描述了一种用于检测 AChE 抑制剂的简单且灵敏的比色测定法的开发。
Hydrolysis of acetylthiocholine mediated by acetylcholine esterase yields the thiol-bearing compound thiocholine. At trace concentrations, thiocholine modulates the growth of Au-Ag nanoparticles on seeding gold nanoparticles in the presence of ascorbic acid. Inhibition of the enzyme by 1,5-bis(4-allyldimethylammoniumphenyl)pentan-3-one dibromide (BW284c51) or by diethyl p-nitrophenyl phosphate (paraoxon) produces lower yields of thiocholine, promoting the catalytic growth of Au-Ag nanoparticles. Here, we describe the development of a simple and sensitive colorimetric assay for the detection of AChE inhibitors.