Direct Observation of the Double-Stranded DNA Formation through Metal Ion-Mediated Base Pairing in the Nanoscale Structure
Direct Observation of the Double-Stranded DNA Formation through Metal Ion-Mediated Base Pairing in the Nanoscale Structure
复制标题
通过纳米级结构中金属离子介导的碱基配对直接观察双链 DNA 的形成
DOI：
10.1002/chem.201805394
复制
发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Endo Masayuki
中科院分区：
文献类型：
--
作者：
Xing Xiwen;Feng Yihong;Yu Zutao;Hidaka Kumi;Liu Fenyong;Ono Akira;Sugiyama Hiroshi;Endo Masayuki
这项工作展示了金属离子诱导DNA纳米结构中双链DNA形成的单分子成像。用C-Ag-C和T-Hg-T金属碱基对研究了DNA折纸框架中金属离子介导的碱基配对的形成。含有连续C或T的靶DNA链被掺入到DNA框架中，结合受金属离子的控制。通过高速原子力显微镜(AFM)观察掺入的DNA链的结构变化来监测双链DNA的形成。利用T-Hg-T碱基对，观察到独特dsDNA的动态形成及其解离。在所设计的纳米结构中，可以看到具有连续T-Hg-T碱基对的不寻常形状的dsDNA的形成。
This work demonstrates single‐molecule imaging of metal‐ion induced double‐stranded DNA formation in DNA nanostructures. The formation of the metal ion‐mediated base pairing in a DNA origami frame was examined using C‐Ag‐C and T‐Hg‐T metallo‐base pairs. The target DNA strands containing consecutive C or T were incorporated into the DNA frame, and the binding was controlled by the addition of metal ions. Double‐stranded DNA formation was monitored by observing the structural changes in the incorporated DNA strands using high‐speed atomic force microscopy (AFM). Using the T‐Hg‐T base pair, the dynamic formation of unique dsDNA and its dissociation were observed. The formation of an unusual shape of dsDNA with consecutive T‐Hg‐T base pairs was visualized in the designed nanoscale structure.